ArticleDigestive diseases and sciences2015
The Relationship Between Colonic Macrophages and MicroRNA-128 in the Pathogenesis of Slow Transit Constipation.
Article in Digestive diseases and sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- The role of gut microbiota in chronic intestinal pseudo-obstruction: exploring fecal microbiota transplantation as a treatment option.Gut microbes · 2026Article
- The Analysis of miRNA-mRNA Network Regulation Revealed the Mechanism of Different Drugs-Induced Constipation in Mice.Biochemical genetics · 2026Article
- MicroRNA regulation of enteric nervous system development and disease.Trends in neurosciences · 2025Review
- Dioscin ameliorates slow transit constipation in mice by up-regulation of the BMP2 secreted by muscularis macrophages.Iranian journal of basic medical sciences · 2022Article
- Characterization of Circular RNA Expression Profiles in Colon Specimens of Patients with Slow Transit Constipation.Disease markers · 2022Article
- Efficacy and MicroRNA-Gut Microbiota Regulatory Mechanisms of Acupuncture for Severe Chronic Constipation: Study Protocol for a Randomized Controlled Trial.Frontiers in medicine · 2022Article
- Role of microRNAs in Disorders of Gut-Brain Interactions: Clinical Insights and Therapeutic Alternatives.Journal of personalized medicine · 2021Review
- Micro-organic basis of functional gastrointestinal (GI) disorders: Role of microRNAs in GI pacemaking cells.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2021Article
- miR-222 regulates cell growth, apoptosis, and autophagy of interstitial cells of Cajal isolated from slow transit constipation rats by targeting c-kit.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2021Article
- The Fecal Microbiota Transplantation: A Remarkable Clinical Therapy for Slow Transit Constipation in Future.Frontiers in cellular and infection microbiology · 2021Review
- Co‑expression network analysis identified specific miRNAs and genes in association with slow‑transit constipation.Molecular medicine reports · 2020Article
- MicroRNA-124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer.Oncology letters · 2019Article
- Functional Disorders: Slow-Transit Constipation.Clinics in colon and rectal surgery · 2017Review
- Role of microRNAs in gastrointestinal smooth muscle fibrosis and dysfunction: novel molecular perspectives on the pathophysiology and therapeutic targeting.American journal of physiology. Gastrointestinal and liver physiology · 2016Review
- Understanding the Pathogenesis of Slow-Transit Constipation: One Step Forward.Digestive diseases and sciences · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent evidence suggests that colonic macrophages and microRNAs play important roles in motor activity in the gastrointestinal tract. However, there are almost no data concerning colonic macrophages and microRNAs in slow transit constipation.
aimThe purpose of this study was to investigate colonic macrophages and microRNA-128 expression in the pathogenesis of slow transit constipation in colon tissues.
methodsFull-thickness colonic specimens from patients undergoing surgery for slow transit constipation, due to refractoriness to other therapeutic interventions (n = 25), were compared to controls (n = 25), and the number of colonic macrophages (as evaluated by specific monoclonal antibodies) was counted. Gene expression analysis of microRNA-128 was performed by microRNA microarray and qRT-PCR. Lastly, bioinformatics analysis, coupled with luciferase reporter assays, was used to investigate the mRNA transcript(s) targeted by microRNA-128.
resultsCompared to controls, 20 of 25 slow transit constipation patients (80 %) had significantly higher numbers of macrophages in colonic specimens, coupled with down-regulation of microRNA-128. Linear regression analyses showed a significant negative correlation between macrophage number and microRNA-128 expression level. Among 83 bioinformatically predicated candidates, mitogen-activated protein kinase 14 (p38α) was validated to be a direct target of microRNA-128 in human intestinal epithelial cells.
conclusionsThis study presents evidence for the negative correlation of macrophage number and microRNA-128 expression, in slow transit constipation patients, representing a possible mechanism of impaired gastrointestinal motility.
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