Evidence map›Paper›PMID 25749876›Full record

ArticleJournal of dental research2015

Wnt5a/Ror2 mediates temporomandibular joint subchondral bone remodeling.

T Yang, J Zhang, Y Cao, M Zhang, L Jing, K Jiao, S Yu, W Chang, D Chen, M Wang

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 48 citations in OpenAlex.

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  19. Molecular signaling in temporomandibular joint osteoarthritis.Journal of orthopaedic translation · 2022
    Review
  20. Role of osteoclast differentiation in the occurrence of osteoarthritis of temporomandibular joint.Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

T YangState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China Department of Stomatology, Chinese PLA General Hospital, Beijing, China.
J ZhangState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Y CaoDepartment of Cardiac Surgery, Air Force General Hospital, PLA, Beijing, China.
M ZhangState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
L JingState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
K JiaoState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
S YuState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
W ChangEndocrine Research Unit, Department of Veterans Affairs Medical Center, Department of Medicine, University of California, San Francisco, CA, USA.
D ChenDepartment of Biochemistry, Rush University Medical Center, Chicago, IL, USA.
M WangState Key Laboratory of Military Stomatology, Department of Oral Anatomy and Physiology, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China mqwang@fmmu.edu.cn.
Air Force Medical University · CNAir Force General Hospital PLA · CNChinese PLA General Hospital · CNRush University Medical Center · USUniversity of California, San Francisco · US

Funding

UCSF Core Center for Musculoskeletal Biology and MedicineP30AR066262 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOTZ, JEFFREY C. · 2014 to 2018
$3.0M
The Mechanism through which TGF-Beta Maintains Chondrocytes in Proliferating StagR01AR054465 · NIAMS · UNIVERSITY OF ROCHESTER · PI CHEN, DI · 2007 to 2018
$2.7M
Pain Mechanisms of Knee Joint OsteoarthritisR01AR062136 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI CHEN, DI · 2012 to 2016
$1.7M
Allosteric Small Molecule Inhibitor Of Nerve Growth Factor Signaling in Low Back PainR21AR067935 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI CHEN, DI · 2015 to 2016
$375k
The Role of CaSR and GABA-B-R in Neuronal Responses to Ischemic Brain InjuryI01BX001960 · VA · VETERANS AFFAIRS MED CTR SAN FRANCISCO · PI CHANG, WENHAN · 2013 to 2016
–
BLRD VA I01 BX001960NIAMS NIH HHS P30 AR066262NIAMS NIH HHS R01 AR054465NIAMS NIH HHS R01 AR062136NIAMS NIH HHS R21 AR067935
6 · The paper itself

Abstract

Increased subchondral trabecular bone turnover due to imbalanced bone-resorbing and bone-forming activities is a hallmark of osteoarthritis (OA). Wnt5a/Ror2 signaling, which can derive from bone marrow stromal cells (BMSCs), takes a role in modulating osteoblast and osteoclast formation. We showed previously that experimentally unilateral anterior crossbites (UACs) elicited OA-like lesions in mice temporomandibular joints (TMJs), displaying as subchondral trabecular bone loss. Herein, we tested the role of BMSC-derived Wnt5a/Ror2 signaling in regulating osteoclast precursor migration and differentiation in this process. The data confirmed the decreased bone mass, increased tartrate-resistant acid phosphatase (TRAP)-positive cell number, and enhanced osteoclast activity in TMJ subchondral trabecular bone of UAC-treated rats. Interestingly, the osteoblast activity in the tissue of TMJ subchondral trabecular bone of these UAC-treated rats was also enhanced, displaying as upregulated expressions of osteoblast markers and increased proliferation, migration, and differentiation capabilities of the locally isolated BMSCs. These BMSCs showed an increased CXCL12 protein expression level and upregulated messenger RNA expressions of Rankl, Wnt5a, and Ror2. Ex vivo data showed that their capacities of inducing migration and differentiation of osteoclast precursors were enhanced, and these enhanced capabilities were restrained after blocking their Ror2 signaling using small interfering RNA (siRNA) assays. Reducing Ror2 expression in the BMSC cell line by siRNA or blocking the downstream signalings with specific inhibitors also demonstrated a suppression of the capacity of the BMSC cell line to promote Wnt5a-dependent migration (including SP600125 and cyclosporine A) and differentiation (cyclosporine A only) of osteoclast precursors. These findings support the idea that Wnt5a/Ror2 signaling in TMJ subchondral BMSCs enhanced by UAC promoted BMSCs to increase Cxcl12 and Rankl expression, in which JNK and/or Ca(2+)/NFAT pathways were involved and therefore were engaged in enhancing the migration and differentiation of osteoclast precursors, leading to increased osteoclast activity and an overall TMJ subchondral trabecular bone loss in the UAC-treated rats.

Indexed as

Acid PhosphataseAnimalsAnthracenesBone DensityBone RemodelingCell DifferentiationCell MovementCell ProliferationChemokine CXCL12Coculture TechniquesCyclosporineEnzyme InhibitorsFemaleIsoenzymesMalocclusionMAP Kinase Signaling SystemAcid PhosphataseAcp5 protein, mouseAnthracenesChemokine CXCL12CXCL12 protein, ratCyclosporineEnzyme InhibitorsIsoenzymesNFATC Transcription FactorspyrazolanthroneRANK LigandReceptor Tyrosine Kinase-like Orphan ReceptorsRor2 protein, ratTartrate-Resistant Acid PhosphataseWnt-5a ProteinWnt5a protein, ratWnt Proteinsbone marrow stromal cellsbone resorptionmandibular condyleosteoarthritisosteoclasttraumatic dental occlusion

Identifiers

PMID25749876
PMCPMC6728679
OpenAlexW1973254856

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.