ArticleAllergy, asthma & immunology research2015
Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach.
Article in Allergy, asthma & immunology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Evaluating Entomopathogenic Nematodes as Biocontrol Agents Against Two Major Cockroach Species,Pathogens (Basel, Switzerland) · 2025Article
- Airborne indoor allergen serine proteases and their contribution to sensitisation and activation of innate immunity in allergic airway disease.European respiratory review : an official journal of the European Respiratory Society · 2024Review
- Intestinal Parasites Associated with American Cockroach (Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- First report ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species.International journal of molecular sciences · 2020Article
- Domiciliary Cockroaches as Carriers of Human Intestinal Parasites in Lagos Metropolis, Southwest Nigeria: Implications for Public Health.Journal of arthropod-borne diseases · 2018Article
- dNP2-ctCTLA-4 inhibits German cockroach extract-induced allergic airway inflammation and hyper-responsiveness via inhibition of Th2 responses.Experimental & molecular medicine · 2017Article
- New Insights into Cockroach Allergens.Current allergy and asthma reports · 2017Review
- CpG Oligodeoxynucleotide Inhibits Cockroach-Induced Asthma via Induction of IFN-γ⁺ Th1 Cells or Foxp3⁺ Regulatory T Cells in the Lung.Allergy, asthma & immunology research · 2016Article
- Cockroach allergen exposure and risk of asthma.Allergy · 2016Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeCockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease.
methodsA cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA.
resultsThe deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract.
conclusionsA novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.
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