Evidence map›Paper›PMID 25749683›Full record

ArticleMolecules (Basel, Switzerland)2015

Tethering in RNA: an RNA-binding fragment discovery tool.

Kiet Tran, Michelle R Arkin, Peter A Beal

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Fragment-Based Approaches to Identify RNA Binders.Journal of medicinal chemistry · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Kiet TranDepartment of Chemistry, University of California, One Shields Ave, Davis, CA 95616, USA. ktutran@ucdavis.edu.
Michelle R ArkinSmall Molecule Discovery Center, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158, USA. Michelle.Arkin@ucsf.edu.
Peter A BealDepartment of Chemistry, University of California, One Shields Ave, Davis, CA 95616, USA. pabeal@ucdavis.edu.
University of California, Davis · USUniversity of California, San Francisco · US

Funding

The Bioorganic Chemistry Of RNA Editing By ADARSR01GM061115 · NIGMS · UNIVERSITY OF UTAH · PI BEAL, PETER A. · 2001 to 2020
$4.5M
Nucleobase analogs for next generation siRNAsR01GM080784 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BEAL, PETER A. · 2007 to 2020
$3.5M
NIGMS NIH HHS R01 GM061115NIGMS NIH HHS R01 GM080784
6 · The paper itself

Abstract

Tethering has been extensively used to study small molecule interactions with proteins through reversible disulfide bond forming reactions to cysteine residues. We describe the adaptation of Tethering to the study of small molecule binding to RNA using a thiol-containing adenosine analog (ASH). Among 30 disulfide-containing small molecules screened for efficient Tethering to ASH-bearing RNAs derived from pre-miR21, a benzotriazole-containing compound showed prominent adduct formation and selectivity for one of the RNAs tested. The results of this screen demonstrate the viability of using thiol-modified nucleic acids to discover molecules with binding affinity and specificity for the purpose of therapeutic compound lead discovery.

Indexed as

Binding SitesDisulfidesHumansMicroRNAsModels, MolecularPharmaceutical PreparationsRNADisulfidesMicroRNAsMIRN21 microRNA, humanPharmaceutical PreparationsRNA

Identifiers

PMID25749683
PMCPMC4760646
OpenAlexW2031770960

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.