ArticlePloS one2015
Improved development of somatic cell cloned mouse embryos by vitamin C and latrunculin A.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Incomplete activation ofLife science alliance · 2023Article
- Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer.International journal of molecular sciences · 2022Review
- Epigenetic manipulation to improve mouse SCNT embryonic development.Frontiers in genetics · 2022Review
- Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming.Theriogenology · 2020Article
- Lessons Learned from Somatic Cell Nuclear Transfer.International journal of molecular sciences · 2020Review
- Endoplasmic Reticulum (ER) Stress Inhibitor or Antioxidant Treatments during Micromanipulation Can Inhibit Both ER and Oxidative Stresses in Porcine SCNT Embryos.Development & reproduction · 2020Article
- Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst.The Journal of biological chemistry · 2019Article
- Transcriptome Analyses Reveal Effects of Vitamin C-Treated Donor Cells on Cloned Bovine Embryo Development.International journal of molecular sciences · 2019Article
- Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states.Epigenetics & chromatin · 2019Article
- Expression of polo-like kinase 1 in pre-implantation stage murine somatic cell nuclear transfer embryos.Journal of veterinary science · 2019Article
- Single blastomeres as a source of mouse embryonic stem cells: effect of genetic background, medium supplements, and signaling modulators on derivation efficiency.Journal of assisted reproduction and genetics · 2019Article
- Potential application of cell reprogramming techniques for cancer research.Cellular and molecular life sciences : CMLS · 2019Review
- Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes.Scientific reports · 2018Article
- Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules.Biology open · 2017Article
- Reprogramming Enhancers in Somatic Cell Nuclear Transfer, iPSC Technology, and Direct Conversion.Stem cell reviews and reports · 2017Review
- Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species.Journal of animal science and technology · 2017Review
- The epigenetic role of vitamin C in health and disease.Cellular and molecular life sciences : CMLS · 2016Review
- Genome-Wide Dynamic Profiling of Histone Methylation during Nuclear Transfer-Mediated Porcine Somatic Cell Reprogramming.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Impaired development of embryos produced by somatic cell nuclear transfer (SCNT) is mostly associated with faulty reprogramming of the somatic nucleus to a totipotent state and can be improved by treatment with epigenetic modifiers. Here we report that addition of 100 μM vitamin C (VitC) to embryo culture medium for at least 16 h post-activation significantly increases mouse blastocyst formation and, when combined with the use of latrunculin A (LatA) during micromanipulation and activation procedures, also development to term. In spite of this, no significant effects on pluripotency (OCT4 and NANOG) or nuclear reprogramming markers (H3K14 acetylation, H3K9 methylation and DNA methylation and hydroxymethylation) could be detected. The use of LatA alone significantly improved in vitro development, but not full-term development. On the other hand, the simultaneous treatment of cloned embryos with VitC and the histone deacetylase inhibitor psammaplin A (PsA), in combination with the use of LatA, resulted in cloning efficiencies equivalent to those of VitC or PsA treatments alone, and the effects on pluripotency and nuclear reprogramming markers were less evident than when only the PsA treatment was applied. These results suggest that although both epigenetic modifiers improve cloning efficiencies, possibly through different mechanisms, they do not show an additive effect when combined. Improvement of SCNT efficiency is essential for its applications in reproductive and therapeutic cloning, and identification of molecules which increase this efficiency should facilitate studies on the mechanism of nuclear reprogramming and acquisition of totipotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.