ArticlePLoS genetics2015
A conserved domain in the scc3 subunit of cohesin mediates the interaction with both mcd1 and the cohesin loader complex.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 62 citations in OpenAlex.
- Crosstalk between cohesins and axis proteins determines meiotic chromosome architecture in Sordaria macrospora.PLoS genetics · 2025Article
- Aberrant cohesin function in Saccharomyces cerevisiae activates Mcd1 degradation to promote cell lethality.PLoS genetics · 2025Article
- Aberrant cohesin function inbioRxiv : the preprint server for biology · 2025Article
- NIPBL and STAG1 enable loop extrusion by providing differential DNA-cohesin affinity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Fdo1, Fkh1, Fkh2, and the Swi6-Mbp1 MBF complex regulate Mcd1 levels to impact eco1 rad61 cell growth in Saccharomyces cerevisiae.Genetics · 2024Article
- Article
- Sororin is an evolutionary conserved antagonist of WAPL.Nature communications · 2024Article
- The N-terminus of Stag1 is required to repress the 2C program by maintaining rRNA expression and nucleolar integrity.Stem cell reports · 2023Article
- Article
- Cohesin: an emerging master regulator at the heart of cardiac development.Molecular biology of the cell · 2023Article
- Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.eLife · 2023Article
- A toolbox for systematic discovery of stable and transient protein interactors in baker's yeast.Molecular systems biology · 2023Article
- Fold-change of chromatin condensation in yeast is a conserved property.Scientific reports · 2022Article
- Chromosome loading of cohesin depends on conserved residues in Scc3.Current genetics · 2021Article
- De Novo Mutation and Rapid Protein (Co-)evolution during Meiotic Adaptation in Arabidopsis arenosa.Molecular biology and evolution · 2021Article
- PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.Cell cycle (Georgetown, Tex.) · 2020Article
- Promotion of Hyperthermic-Induced rDNA Hypercondensation inGenetics · 2020Article
- Evolutionary repair: Changes in multiple functional modules allow meiotic cohesin to support mitosis.PLoS biology · 2020Article
- Monomeric cohesin state revealed by live-cell single-molecule spectroscopy.EMBO reports · 2020Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Structural Maintenance of Chromosome (SMC) complex, termed cohesin, is essential for sister chromatid cohesion. Cohesin is also important for chromosome condensation, DNA repair, and gene expression. Cohesin is comprised of Scc3, Mcd1, Smc1, and Smc3. Scc3 also binds Pds5 and Wpl1, cohesin-associated proteins that regulate cohesin function, and to the Scc2/4 cohesin loader. We mutagenized SCC3 to elucidate its role in cohesin function. A 5 amino acid insertion after Scc3 residue I358, or a missense mutation of residue D373 in the adjacent stromalin conservative domain (SCD) induce inviability and defects in both cohesion and cohesin binding to chromosomes. The I358 and D373 mutants abrogate Scc3 binding to Mcd1. These results define an Scc3 region extending from I358 through the SCD required for binding Mcd1, cohesin localization to chromosomes and cohesion. Scc3 binding to the cohesin loader, Pds5 and Wpl1 are unaffected in I358 mutant and the loader still binds the cohesin core trimer (Mcd1, Smc1 and Smc3). Thus, Scc3 plays a critical role in cohesin binding to chromosomes and cohesion at a step distinct from loader binding to the cohesin trimer. We show that residues Y371 and K372 within the SCD are critical for viability and chromosome condensation but dispensable for cohesion. However, scc3 Y371A and scc3 K372A bind normally to Mcd1. These alleles also provide evidence that Scc3 has distinct mechanisms of cohesin loading to different loci. The cohesion-competence, condensation-incompetence of Y371 and K372 mutants suggests that cohesin has at least one activity required specifically for condensation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.