ArticleScientific reports2015
In vivo quantification of the structural changes of collagens in a melanoma microenvironment with second and third harmonic generation microscopy.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Illuminating aging with multimodal optical metabolic imaging.Science advances · 2026Review
- Second harmonic generation for brain imaging: pathology-related studies.Biophysical reviews · 2025Article
- Three-dimensional quantitative evaluation of hypertension-induced aortic fibre remodelling based on multiphoton microscopy: a cross-age perspective.Royal Society open science · 2025Article
- Assessing Wound Healing in Vivo Using a Dual-Function Phosphorescent Probe Sensitive to Tissue Oxygenation and Regenerating Collagen.ACS applied materials & interfaces · 2025Article
- QuantifyingBiophotonics discovery · 2024Article
- Targeting the secreted RGDKGE collagen fragment reduces PD‑L1 by a proteasome‑dependent mechanism and inhibits tumor growth.Oncology reports · 2023Article
- In vivo tumor immune microenvironment phenotypes correlate with inflammation and vasculature to predict immunotherapy response.Nature communications · 2022Article
- Noninvasive assessment of liver function reserve with fluorescent dosimetry of indocyanine green.Biomedical optics express · 2022Article
- Remodelling of the fibre-aggregate structure of collagen gels by cancer-associated fibroblasts: A time-resolved grey-tone image analysis based on stochastic modelling.Frontiers in immunology · 2022Article
- Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing.Communications biology · 2021Article
- Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021Review
- Imaging methods to evaluate tumor microenvironment factors affecting nanoparticle drug delivery and antitumor response.Cancer drug resistance (Alhambra, Calif.) · 2021Article
- Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies.Scientific reports · 2020Article
- Collagen fiber structure guides 3D motility of cytotoxic T lymphocytes.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
- Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.Journal of biophotonics · 2019Article
- Tensor regularized total variation for denoising of third harmonic generation images of brain tumors.Journal of biophotonics · 2019Article
- Types of advanced optical microscopy techniques for breast cancer research: a review.Lasers in medical science · 2018Review
- Complex mechanics of the heterogeneous extracellular matrix in cancer.Extreme Mechanics Letters · 2018Review
- Discovering Macrophage Functions UsingFrontiers in immunology · 2018Review
- Characterization of the Tumor Microenvironment and Tumor-Stroma Interaction by Non-invasive Preclinical Imaging.Frontiers in oncology · 2017Review
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4 authors.
Funding
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Abstract
Using in vivo second harmonic generation (SHG) and third harmonic generation (THG) microscopies, we tracked the course of collagen remodeling over time in the same melanoma microenvironment within an individual mouse. The corresponding structural and morphological changes were quantitatively analyzed without labeling using an orientation index (OI), the gray level co-occurrence matrix (GLCM) method, and the intensity ratio of THG to SHG (RTHG/SHG). In the early stage of melanoma development, we found that collagen fibers adjacent to a melanoma have increased OI values and SHG intensities. In the late stages, these collagen networks have more directionality and less homogeneity. The corresponding GLCM traces showed oscillation features and the sum of squared fluctuation VarGLCM increased with the tumor sizes. In addition, the THG intensities of the extracellular matrices increased, indicating an enhanced optical inhomogeneity. Multiplying OI, VarGLCM, and RTHG/SHG together, the combinational collagen remodeling (CR) index at 4 weeks post melanoma implantation showed a 400-times higher value than normal ones. These results validate that our quantitative indices of SHG and THG microscopies are sensitive enough to diagnose the collagen remodeling in vivo. We believe these indices have the potential to help the diagnosis of skin cancers in clinical practice.
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