ArticleThe American journal of pathology2015
Ultrasound-guided intrauterine injection of lipopolysaccharide as a novel model of preterm birth in the mouse.
Article in The American journal of pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Interventions for Infection and Inflammation-Induced Preterm Birth: a Preclinical Systematic Review.Reproductive sciences (Thousand Oaks, Calif.) · 2023Pooled it
- Arachidonic Acid-Induced Contraction of Smooth Muscle Is Mediated by MLC Phosphorylation in Preterm Birth.Journal of pregnancy · 2026Article
- N,N-dimethylacetamide blocks inflammation-induced preterm birth and remediates maternal systemic immune responses.Scientific reports · 2025Article
- N,N-dimethylacetamide blocks inflammation-induced preterm birth and remediates maternal systemic immune responses.bioRxiv : the preprint server for biology · 2025Article
- Impaired SREBP1-mediated regulation of lipid metabolism promotes inflammation in chronic endometritis.Frontiers in immunology · 2025Article
- Ultrasound-guided hepatic portal vein injection is not a reproducible technique for delivery of cell therapies to the liver in mice.Diabetic medicine : a journal of the British Diabetic Association · 2023Article
- Ultrasound Guided Surgery as a Refinement Tool in Oncology Research.Animals : an open access journal from MDPI · 2022Review
- Microbiota of the Pregnant Mouse: Characterization of the Bacterial Communities in the Oral Cavity, Lung, Intestine, and Vagina through Culture and DNA Sequencing.Microbiology spectrum · 2022Article
- Insights into the Black Box of Intra-Amniotic Infection and Its Impact on the Premature Lung: From Clinical and Preclinical Perspectives.International journal of molecular sciences · 2022Review
- Review
- Development of a Rat Model of Intra-Amniotic InflammationFrontiers in pharmacology · 2022Article
- Understanding Host-Pathogen Interactions in Acute Chorioamnionitis Through the Use of Animal Models.Frontiers in cellular and infection microbiology · 2021Review
- Maternal regulation of inflammatory cues is required for induction of preterm birth.JCI insight · 2020Article
- Animal models closer to intrauterine adhesive pathology.Annals of translational medicine · 2020Article
- Inflammation-mediated generation and inflammatory potential of human placental cell-free fetal DNA.Placenta · 2020Article
- Characterization of an Adapted Murine Model of Intrauterine Inflammation-Induced Preterm Birth.The American journal of pathology · 2020Article
- Cervical epithelial damage promotes Ureaplasma parvum ascending infection, intrauterine inflammation and preterm birth induction in mice.Nature communications · 2020Article
- Immunobiology of Acute Chorioamnionitis.Frontiers in immunology · 2020Review
- Cervical Gene Delivery of the Antimicrobial Peptide, Human β-Defensin (HBD)-3, in a Mouse Model of Ascending Infection-Related Preterm Birth.Frontiers in immunology · 2020Article
- Cathelicidins and the Onset of Labour.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Mouse models are used to study mechanisms that link intrauterine infection and preterm birth (PTB). To mimic intrauterine infection, lipopolysaccharide (LPS) is commonly injected into the uterus via minilaparotomy, which is invasive, and can cause PTB in control animals. We hypothesized that less-invasive ultrasound-guided intrauterine LPS injection or intravaginal LPS administration could induce PTB by stimulating an inflammatory response of the uteroplacental tissues, while minimizing PTB in control animals. On day 17 of gestation mice received LPS intravaginally (10 to 240 μg; n = 3 to 8) or into the uterus (20 μg) under ultrasound guidance (n = 7) or via laparotomy (n = 7). Control animals received phosphate-buffered saline (PBS; n = 5 to 7). Intrauterine administration of LPS, both under ultrasound guidance and via laparotomy, induced delivery earlier than in PBS control groups (P < 0.01). Intravaginal LPS administration did not stimulate PTB. Quantitative real-time PCR and immunohistochemistry of tissues harvested 6 hours after treatment confirmed that ultrasound-guided LPS administration induced a localized inflammatory response. Ultrasound-guided intrauterine LPS injection reliably induces PTB in the mouse and mimics the local inflammatory and immune responses observed in the more-invasive laparotomy model of inflammation-induced PTB. Ultrasound-guided intrauterine LPS injection is a useful novel model of PTB for future studies and concords with the principles of reduction, replacement, and refinement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.