ArticlePloS one2015
Bacterial diversity associated with wild caught Anopheles mosquitoes from Dak Nong Province, Vietnam using culture and DNA fingerprint.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Bacterial and Fungal Microbiota in Anopheles darlingi Exhibit Differences in Diversity Across Three Main Colombian Malaria-endemic Regions.Microbial ecology · 2026Article
- The type of environment has a greater impact on the larval microbiota of Anopheles arabiensis than on the microbiota of their breeding water.FEMS microbiology ecology · 2025Article
- Mosquito Gut Microbiota: A Review.Pathogens (Basel, Switzerland) · 2024Review
- Characterization of the Tissue and Strain-Specific Microbiota ofTropical medicine and infectious disease · 2024Article
- Characterizations of Larval Gut Bacteria of Anopheles subpictus Grassi (1899) and their Role in Mosquito Development in Hooghly, West Bengal, India.Applied biochemistry and biotechnology · 2022Article
- Isolation, Identification, and Analysis of Potential Functions of Culturable Bacteria Associated with an Invasive Gall Wasp, Leptocybe invasa.Microbial ecology · 2022Article
- Microbiota identified from preserved Anopheles.Malaria journal · 2021Article
- Microbiota Variation Across Life Stages of European Field-CaughtFrontiers in microbiology · 2021Article
- Bacterial Microbiome in Wild-CaughtFrontiers in microbiology · 2020Article
- Isolation and identification of microflora from the midgut and salivary glands of Anopheles species in malaria endemic areas of Ethiopia.BMC microbiology · 2019Article
- Generational conservation of composition and diversity of field-acquired midgut microbiota in Anopheles gambiae (sensu lato) during colonization in the laboratory.Parasites & vectors · 2019Article
- Influence of midgut microbiota in Anopheles stephensi on Plasmodium berghei infections.Malaria journal · 2018Article
- Review
- Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors.Scientific reports · 2018Article
- Micromanaging Immunity in the Murine Host vs. the Mosquito Vector: Microbiota-Dependent Immune Responses to Intestinal Parasites.Frontiers in cellular and infection microbiology · 2018Review
- Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany.Parasitology research · 2017Article
- Culex pipiens and Culex restuans mosquitoes harbor distinct microbiota dominated by few bacterial taxa.Parasites & vectors · 2016Article
- Diversity of the Bacterial Microbiota ofFrontiers in microbiology · 2016Article
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Authors and funding
5 authors.
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Abstract
backgroundMicrobiota of Anopheles midgut can modulate vector immunity and block Plasmodium development. Investigation on the bacterial biodiversity in Anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of Africa. Vietnam is an endemic country for both malaria and Bancroftian filariasis whose parasitic agents can be transmitted by the same Anopheles species. No information on the microbiota of Anopheles mosquitoes in Vietnam was available previous to this study.
methodThe culture dependent approach, using different mediums, and culture independent (16S rRNA PCR - TTGE) method were used to investigate the bacterial biodiversity in the abdomen of 5 Anopheles species collected from Dak Nong Province, central-south Vietnam. Molecular methods, sequencing and phylogenetic analysis were used to characterize the microbiota. RESULTS AND DISCUSSION: The microbiota in wild-caught Anopheles was diverse with the presence of 47 bacterial OTUs belonging to 30 genera, including bacterial genera impacting Plasmodium development. The bacteria were affiliated with 4 phyla, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, the latter being the dominant phylum. Four bacterial genera are newly described in Anopheles mosquitoes including Coxiella, Yersinia, Xanthomonas, and Knoellia. The bacterial diversity per specimen was low ranging from 1 to 4. The results show the importance of pairing culture and fingerprint methods to better screen the bacterial community in Anopheles mosquitoes.
conclusionSampled Anopheles species from central-south Vietnam contained a diverse bacterial microbiota that needs to be investigated further in order to develop new malaria control approaches. The combination of both culture and DNA fingerprint methods allowed a thorough and complementary screening of the bacterial community in Anopheles mosquitoes.
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