Evidence map›Paper›PMID 25747388›Full record

ArticleFEBS letters2015

Acquiring snapshots of the orientation of trans-membrane protein domains using a hybrid FRET pair.

Robert F Gahl, Ephrem Tekle, Gefei Alex Zhu, Justin W Taraska, Nico Tjandra

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Robert F GahlBiochemistry and Biophysics Center, Laboratory of Molecular Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA.
Ephrem TekleBiochemistry and Biophysics Center, Laboratory of Molecular Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA.
Gefei Alex ZhuBiochemistry and Biophysics Center, Laboratory of Molecular Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA.
Justin W TaraskaBiochemistry and Biophysics Center, Laboratory of Molecular Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA.
Nico TjandraBiochemistry and Biophysics Center, Laboratory of Molecular Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA. Electronic address: tjandran@nhlbi.nih.gov.

Funding

Regulation of mitochondria morphologyZIAHL006064 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI TJANDRA, NICO · 2010 to 2025
$20.9M
Intramural NIH HHS ZIA HL006064
6 · The paper itself

Abstract

One challenge in studying the function of membrane-embedded proteins is determining the orientation of key domains in the context of the changing and dynamic membrane environment. We describe a confocal microscopy setup that utilizes external electric field pulses to direct dipicrylamine (DPA) to a membrane leaflet. The detection of FRET between DPA and a fluorescent probe attributes it to the inner or outer leaflet of a membrane. By utilizing short acquisition times and confocal imaging, this attribution could be made even in changing membrane environments. Our setup adds versatility to the study of the biological activity of membrane-embedded proteins.

Indexed as

Fluorescence Resonance Energy TransferAnimalsDNAFluorescent DyesMembrane ProteinsModels, MolecularPC12 CellsProtein Structure, SecondaryProtein Structure, TertiaryRatsDNAFluorescent DyesMembrane ProteinsConfocal microscopyFluorescenceFörster resonance energy transfer (FRET)Giant unilamellar vesicles (GUV)Live cellsMembrane proteins

Identifiers

PMID25747388
PMCPMC4373971

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.