ArticleApplied and environmental microbiology2015
Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 62 citations in OpenAlex.
- The Recent Progress of Tricyclic Aromadendrene-Type Sesquiterpenoids: Biological Activities and Biosynthesis.Biomolecules · 2024Review
- Biosynthesis Progress of High-Energy-Density Liquid Fuels Derived from Terpenes.Microorganisms · 2024Review
- Compartmentalization engineering of yeasts to overcome precursor limitations and cytotoxicity in terpenoid production.Frontiers in bioengineering and biotechnology · 2023Review
- Engineering for life in toxicity: Key to industrializing microbial synthesis of high energy density fuels.Engineering microbiology · 2022Review
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol.Biotechnology for biofuels · 2020Article
- Improvement of sabinene tolerance ofBiotechnology for biofuels · 2020Article
- Sustainable Production of Microbial Isoprenoid Derived Advanced Biojet Fuels Using Different Generation Feedstocks: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Identifying and engineering the ideal microbial terpenoid production host.Applied microbiology and biotechnology · 2019Review
- Evolutionary engineering inBiotechnology for biofuels · 2019Article
- Renewable production of high density jet fuel precursor sesquiterpenes fromBiotechnology for biofuels · 2018Article
- Evolutionary engineering improves tolerance for medium-chain alcohols inBiotechnology for biofuels · 2018Article
- Yeast's balancing act between ethanol and glycerol production in low-alcohol wines.Microbial biotechnology · 2017Review
- Dynamic control of ERG20 expression combined with minimized endogenous downstream metabolism contributes to the improvement of geraniol production in Saccharomyces cerevisiae.Microbial cell factories · 2017Article
- Positive-feedback, ratiometric biosensor expression improves high-throughput metabolite-producer screening efficiency in yeast.Synthetic biology (Oxford, England) · 2017Article
- Biotechnological production of limonene in microorganisms.Applied microbiology and biotechnology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoterpenes are liquid hydrocarbons with applications ranging from flavor and fragrance to replacement jet fuel. Their toxicity, however, presents a major challenge for microbial synthesis. Here we evolved limonene-tolerant Saccharomyces cerevisiae strains and sequenced six strains across the 200-generation evolutionary time course. Mutations were found in the tricalbin proteins Tcb2p and Tcb3p. Genomic reconstruction in the parent strain showed that truncation of a single protein (tTcb3p(1-989)), but not its complete deletion, was sufficient to recover the evolved phenotype improving limonene fitness 9-fold. tTcb3p(1-989) increased tolerance toward two other monoterpenes (β-pinene and myrcene) 11- and 8-fold, respectively, and tolerance toward the biojet fuel blend AMJ-700t (10% cymene, 50% limonene, 40% farnesene) 4-fold. tTcb3p(1-989) is the first example of successful engineering of phase tolerance and creates opportunities for production of the highly toxic C10 alkenes in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.