ReviewThe Journal of molecular diagnostics : JMD2015
A systematic approach to novel virus discovery in emerging infectious disease outbreaks.
Review in The Journal of molecular diagnostics : JMD, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Detection and genetic characterization of pigeon gammacoronaviruses.The Journal of veterinary medical science · 2026Article
- Human Circovirus in Patients with Hepatitis, Hong Kong.Emerging infectious diseases · 2024Article
- Scale-Up of COVID-19 Testing Services in NYC, 2020-2021: Lessons Learned to Maximize Reach, Equity and Timeliness.Journal of urban health : bulletin of the New York Academy of Medicine · 2024Article
- Article
- Automated System for Multiplexing Detection of COVID-19 and Other Respiratory Pathogens.IEEE journal of translational engineering in health and medicine · 2023Article
- Article
- Mapping the viruses belonging to the order Bunyavirales in China.Infectious diseases of poverty · 2022Article
- Human infections with neglected vector-borne pathogens in China: A systematic review.The Lancet regional health. Western Pacific · 2022Review
- Unraveling the viral dark matter through viral metagenomics.Frontiers in immunology · 2022Review
- Nanotechnology: A Potential Weapon to Fight against COVID-19.Particle & particle systems characterization : measurement and description of particle properties and behavior in powders and other disperse systems · 2022Review
- Human Alphacoronavirus Universal Primers for Genome Amplification and Sequencing.Frontiers in microbiology · 2022Article
- Nanobased Platforms for Diagnosis and Treatment of COVID-19: From Benchtop to Bedside.ACS biomaterials science & engineering · 2021Review
- A network-based model to explore the role of testing in the epidemiological control of the COVID-19 pandemic.BMC infectious diseases · 2021Article
- Extending the Enterovirus Lead: Could a Related Picornavirus be Responsible for Diabetes in Humans?Microorganisms · 2020Review
- Molecular detection and characterisation of feline morbillivirus in domestic cats in Malaysia.Veterinary microbiology · 2019Article
- Epidemiological and Clinical Characteristics of Human Hepegivirus 1 Infection in Patients With Hepatitis C.Open forum infectious diseases · 2019Article
- Laboratory Methods in Molecular Epidemiology: Viral Infections.Microbiology spectrum · 2018Review
- Nanomaterial-based biosensors for detection of pathogenic virus.Trends in analytical chemistry : TRAC · 2017Review
- The Human Virome: Implications for Clinical Practice in Transplantation Medicine.Journal of clinical microbiology · 2017Review
- The role of laboratory diagnostics in emerging viral infections: the example of the Middle East respiratory syndrome epidemic.Journal of microbiology (Seoul, Korea) · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The discovery of novel viruses is of great importance to human health-both in the setting of emerging infectious disease outbreaks and in disease syndromes of unknown etiology. Despite the recent proliferation of many efficient virus discovery methods, careful selection of a combination of methods is important to demonstrate a novel virus, its clinical associations, and its relevance in a timely manner. The identification of a patient or an outbreak with distinctive clinical features and negative routine microbiological workup is often the starting point for virus hunting. This review appraises the roles of culture, electron microscopy, and nucleic acid detection-based methods in optimizing virus discovery. Cell culture is generally slow but may yield viable virus. Although the choice of cell line often involves trial and error, it may be guided by the clinical syndrome. Electron microscopy is insensitive but fast, and may provide morphological clues to choice of cell line or consensus primers for nucleic acid detection. Consensus primer PCR can be used to detect viruses that are closely related to known virus families. Random primer amplification and high-throughput sequencing can catch any virus genome but cannot yield an infectious virion for testing Koch postulates. A systematic approach that incorporates carefully chosen combinations of virus detection techniques is required for successful virus discovery.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.