ArticlePloS one2015
Properties of a newly identified esterase from Bacillus sp. K91 and its novel function in diisobutyl phthalate degradation.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 100 citations in OpenAlex.
- Interspecies Exchange of Mobile Genetic Elements During a Plant Disease Outbreak.Genome biology and evolution · 2026Article
- Predicting the plastic biodegradation potential within microbial lineages and across global ecosystems.Microbial genomics · 2026Article
- Bacterial degradation of aromatic ester pollutants in agro-ecosystems: implications for bioremediation.Applied microbiology and biotechnology · 2026Review
- Biological Degradation of Phthalates: From Bioremediation to Plastic Waste Valorization.ChemSusChem · 2026Review
- Biodegradation of plasticizer benzyl butyl phthalate by Paenarthrobacter sp. strain M76 in liquid culture and soil microcosms.Biodegradation · 2026Article
- Cloning and Characterization of GDSL Esterases fromBiology · 2026Article
- Identification and Characterization of a Novel Di-(2-ethylhexyl) Phthalate Hydrolase from a Marine Bacterial StrainInternational journal of molecular sciences · 2025Article
- Identification and characterisation of moderately thermostable diisobutyl phthalate degrading esterase from a Great Artesian BasinBiotechnology reports (Amsterdam, Netherlands) · 2024Article
- Biodegradation of DEP, DIBP, and BBP by a psychrotolerant Sphingobium yanoikuyae strain P4: Degradation potentiality and mechanism study.Archives of microbiology · 2024Article
- Whole-genome analysis of3 Biotech · 2023Article
- Biochemical and Multi-Omics Approaches To Obtain Molecular Insights into the Catabolism of the Plasticizer Benzyl Butyl Phthalate inMicrobiology spectrum · 2023Article
- Evaluation of distinct molecular architectures and coordinated regulation of the catabolic pathways of oestrogenic dioctyl phthalate isomers inMicrobiology (Reading, England) · 2023Article
- Phthalate Esters Metabolic StrainMicroorganisms · 2022Article
- Enzyme catalyzes ester bond synthesis and hydrolysis: The key step for sustainable usage of plastics.Frontiers in microbiology · 2022Review
- Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli.Microbial cell factories · 2020Article
- Characterization of a novel family VIII esterase EstM2 from soil metagenome capable of hydrolyzing estrogenic phthalates.Microbial cell factories · 2020Article
- Synergistic Degradation of Pyrethroids by the Quorum Sensing-Regulated Carboxylesterase ofFrontiers in bioengineering and biotechnology · 2020Article
- Article
- Bacteria-mediated phthalic acid esters degradation and related molecular mechanisms.Applied microbiology and biotechnology · 2018Review
- Characterization and Genome Analysis of a Phthalate Esters-Degrading StrainBioMed research international · 2018Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The widely used plasticizer phthalate esters (PAEs) have become a public concern because of their effects on environmental contamination and toxicity on mammals. However, the biodegradation of PAEs, especially diisobutyl phthalate (DiBP), remains poorly understood. In particular, genes involved in the hydrolysis of these compounds were not conclusively identified. In this study, the CarEW gene, which encodes an enzyme that is capable of hydrolyzing ρ-nitrophenyl esters of fatty acids, was cloned from a thermophilic bacterium Bacillus sp. K91 and heterologously expressed in Escherichia coli BL21 using the pEASY-E2 expression system. The enzyme showed a monomeric structure with a molecular mass of approximately 53.76 kDa and pI of 4.88. The enzyme exhibited maximal activity at pH 7.5 and 45 °C, with ρ-NP butyrate as the best substrate. The enzyme was fairly stable within the pH range from 7.0 to 8.5. High-pressure liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS) were employed to detect the catabolic pathway of DiBP. Two intermediate products were identified, and a potential biodegradation pathway was proposed. Altogether, our findings present a novel DiBP degradation enzyme and indicate that the purified enzyme may be a promising candidate for DiBP detoxification and for environmental protection.
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