ArticleThe Journal of biological chemistry2015
Trans-sulfuration Pathway Seleno-amino Acids Are Mediators of Selenomethionine Toxicity in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Selenium and Type 2 Diabetes: Systematic Review.Nutrients · 2018Pooled it
- Selenoproteins: Minute yet vital players governing cellular fate.Genes & diseases · 2026Review
- Vascular Aging and Atherosclerosis: The Modulatory Impact of Selenium-A Comprehensive Review.Cells · 2026Review
- Protic small molecule bioregulators.Redox biology · 2025Review
- Predictors of Plasma Selenium Levels and Association with Prognosis in Outpatients with Heart Failure: a 36-Month Prospective Cohort Study.Biological trace element research · 2025Article
- Exposure to Selenomethionine and Selenocystine Induces Redox-Mediated ER Stress in Normal Breast Epithelial MCF-10A Cells.Biological trace element research · 2025Article
- Clinical and mechanistic insights into biomedical application of Se-enriched probiotics and biogenic selenium nanoparticles.Biotechnology letters · 2025Review
- Biosynthesis, Engineering, and Delivery of Selenoproteins.International journal of molecular sciences · 2023Review
- Recoding UAG to selenocysteine inRNA (New York, N.Y.) · 2023Article
- Metabolism and Anticancer Mechanisms of Selocompounds: Comprehensive Review.Biological trace element research · 2023Review
- Time-Resolved Examination of Fungal Selenium Redox Transformations.ACS earth & space chemistry · 2023Article
- Enhanced selenocysteine biosynthesis for seleno-methylselenocysteine production in Bacillus subtilis.Applied microbiology and biotechnology · 2023Article
- Reactive sulfur and selenium species in the regulation of bone homeostasis.Free radical biology & medicine · 2022Review
- Reasons for 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde resistance in Saccharomyces cerevisiae: current state of knowledge and perspectives for further improvements.Applied microbiology and biotechnology · 2021Review
- Methylselenol Produced In Vivo from Methylseleninic Acid or Dimethyl Diselenide Induces Toxic Protein Aggregation inInternational journal of molecular sciences · 2021Article
- Pathway-based signature transcriptional profiles as tolerance phenotypes for the adapted industrial yeast Saccharomyces cerevisiae resistant to furfural and HMF.Applied microbiology and biotechnology · 2020Article
- Selenium and at-risk pregnancy: challenges and controversies.Thyroid research · 2020Review
- A cysteinyl-tRNA synthetase variant confers resistance against selenite toxicity and decreases selenocysteine misincorporation.The Journal of biological chemistry · 2019Article
- A practical method for efficient and optimal production of Seleno-methionine-labeled recombinant protein complexes in the insect cells.Protein science : a publication of the Protein Society · 2019Article
- SEXUAL DIMORPHISM IN SELENIUM METABOLISM AND SELENOPROTEINS.Free radical biology & medicine · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxicity of selenomethionine, an organic derivative of selenium widely used as supplement in human diets, was studied in the model organism Saccharomyces cerevisiae. Several DNA repair-deficient strains hypersensitive to selenide displayed wild-type growth rate properties in the presence of selenomethionine indicating that selenide and selenomethionine exert their toxicity via distinct mechanisms. Cytotoxicity of selenomethionine decreased when the extracellular concentration of methionine or S-adenosylmethionine was increased. This protection resulted from competition between the S- and Se-compounds along the downstream metabolic pathways inside the cell. By comparing the sensitivity to selenomethionine of mutants impaired in the sulfur amino acid pathway, we excluded a toxic effect of Se-adenosylmethionine, Se-adenosylhomocysteine, or of any compound in the methionine salvage pathway. Instead, we found that selenomethionine toxicity is mediated by the trans-sulfuration pathway amino acids selenohomocysteine and/or selenocysteine. Involvement of superoxide radicals in selenomethionine toxicity in vivo is suggested by the hypersensitivity of a Δsod1 mutant strain, increased resistance afforded by the superoxide scavenger manganese, and inactivation of aconitase. In parallel, we showed that, in vitro, the complete oxidation of the selenol function of selenocysteine or selenohomocysteine by dioxygen is achieved within a few minutes at neutral pH and produces superoxide radicals. These results establish a link between superoxide production and trans-sulfuration pathway seleno-amino acids and emphasize the importance of the selenol function in the mechanism of organic selenium toxicity.
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Registered trials
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