Evidence map›Paper›PMID 25743694›Full record

ReviewCell and tissue research2015

Advances in chemical labeling of proteins in living cells.

Qi Yan, Marcel P Bruchez

Abstract readReview
In one paragraph

Review in Cell and tissue research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021
    Review
  8. Review
  9. Single-molecule localization microscopy.Nature reviews. Methods primers · 2021
    Article
  10. Review
  11. Review
  12. Article
  13. Super-resolution microscopy demystified.Nature cell biology · 2019
    Review
  14. Review
  15. Review
  16. Article
  17. IL-6-HaloTagJournal of biological methods · 2017
    Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Qi YanSharp Edge Laboratories, Pittsburgh, Pa., USA.
Marcel P Bruchez

Funding

Targeted fluorescent indicators for endothelial physiology: Ca(II), ROS, NOR01EB017268 · NIBIB · CARNEGIE-MELLON UNIVERSITY · PI BRUCHEZ, MARCEL P, ST CROIX, CLAUDETTE MARIE · 2013 to 2016
$2.5M
SINGLE MOLECULE DETECTION OF ION CHANNELS IN NEURONSR21MH100612 · NIMH · CARNEGIE-MELLON UNIVERSITY · PI BARTH, ALISON L, BRUCHEZ, MARCEL P · 2013 to 2014
$420k
NIBIB NIH HHS R01 EB017268NIMH NIH HHS R21 MH100612
6 · The paper itself

Abstract

The pursuit of quantitative biological information via imaging requires robust labeling approaches that can be used in multiple applications and with a variety of detectable colors and properties. In addition to conventional fluorescent proteins, chemists and biologists have come together to provide a range of approaches that combine dye chemistry with the convenience of genetic targeting. This hybrid-tagging approach amalgamates the rational design of properties available through synthetic dye chemistry with the robust biological targeting available with genetic encoding. In this review, we discuss the current range of approaches that have been exploited for dye targeting or for targeting and activation and some of the recent applications that are uniquely permitted by these hybrid-tagging approaches.

Indexed as

AnimalsCell SurvivalFluorescent DyesHumansPeptidesProteinsStaining and LabelingFluorescent DyesPeptidesProteins

Identifiers

PMID25743694
PMCPMC4380784

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.