Evidence map›Paper›PMID 25743476›Full record

ArticlePathogens and disease2015

Clostridium difficile toxin B intoxicated mouse colonic epithelial CT26 cells stimulate the activation of dendritic cells.

Tuxiong Huang, Gregorio Perez-Cordon, Lianfa Shi, Guangchao Li, Xingmin Sun, Xiaoning Wang, Jufang Wang, Hanping Feng

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens and disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Host Immune Responses toFrontiers in microbiology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Tuxiong HuangSchool of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou 51006, China Department of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, MD 21021, USA.
Gregorio Perez-CordonDepartment of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, MD 21021, USA.
Lianfa ShiDepartment of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, MD 21021, USA.
Guangchao LiSchool of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou 51006, China.
Xingmin SunDepartment of Infectious Disease and Global Health, Tufts Cummings School of Veterinary Medicine, North Grafton, MA 01536, USA.
Xiaoning WangInstitute of Life Science, General Hospital of the People's Liberation Army, Beijing 100853, China.
Jufang WangSchool of Bioscience and Bioengineering, South China University of Technology (SCUT), Guangzhou 51006, China.
Hanping FengDepartment of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, MD 21021, USA hfeng@umaryland.edu.
South China University of Technology · CNUniversity of Maryland, Baltimore · USNew England Disabled Sports · USPeople's Liberation Army No. 150 Hospital · CN

Funding

Shigella Live Vector-Based Multivalent VaccineU19AI109776 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI LEVINE, MYRON MAX · 2014 to 2018
$25.4M
Development of Vaccines against Clostridium difficile InfectionR01AI088748 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI FENG, HANPING, TZIPORI, SAUL R · 2010 to 2014
$3.1M
Epithelium, dendritic cells, and Clostridium difficile associated colitisR01DK084509 · NIDDK · UNIVERSITY OF MARYLAND BALTIMORE · PI FENG, HANPING · 2010 to 2014
$1.7M
Toxemia and systemic disease in Clostridium difficile infectionR56AI099458 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI FENG, HANPING · 2013 to 2013
$388k
NIAID NIH HHS R01 AI088748NIAID NIH HHS R01AI088748NIAID NIH HHS R56 AI099458NIAID NIH HHS R56AI99458NIAID NIH HHS U19 AI109776NIDDK NIH HHS R01 DK084509NIDDK NIH HHS R01DK084509
6 · The paper itself

Abstract

Clostridium difficile causes antibiotic-associated diarrhea and pseudomembranous colitis mainly through two exotoxins TcdA and TcdB that target intestinal epithelial cells. Dendritic cells (DCs) play an important role in regulating intestinal inflammatory responses. In the current study, we explored the interaction of TcdB-intoxicated epithelial cells with mouse bone marrow-derived DCs. TcdB induced cell death and heat shock protein translocation in mouse intestinal epithelial CT26 cells. The intoxicated epithelial cells promoted the phagocytosis and the TNF-α secretion by DCs. Incubation with TcdB-intoxicated CT26 cells stimulated DC maturation. Moreover, TcdB-treated CT26 cells induced DC immigration when they were injected into mice subcutaneously. Taken together, these data demonstrate that TcdB-intoxicated intestinal epithelial cells are able to stimulate DC activation in vitro and attract DCs in vivo, indicating that epithelial cells may be able to regulate DC activation under the exposure of TcdB during C. difficile infection.

Indexed as

Cell CommunicationAnimalsBacterial ProteinsBacterial ToxinsCell DeathCell DifferentiationCell MovementDendritic CellsEpithelial CellsHeat-Shock ProteinsMaleMice, Inbred BALB CPhagocytosisProtein TransportTumor Necrosis Factor-alphaBacterial ProteinsBacterial ToxinsHeat-Shock ProteinstoxB protein, Clostridium difficileTumor Necrosis Factor-alphaClostridium difficiledendritic cellsepithelial cellsinflammationTcdB

Identifiers

PMID25743476
PMCPMC4435672
OpenAlexW1975938739

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.