ArticlePloS one2015
An ex vivo model for anti-angiogenic drug testing on intact microvascular networks.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Predicting red blood cell transport and capillary hemodynamics in angiogenic and tumor vascular networks in silico.Biophysical journal · 2025Article
- Time-Lapse Observation of Cell Dynamics During Angiogenesis Using the Rat Mesentery Culture Model.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Variations in mechanical stiffness alter microvascular sprouting and stability in a PEG hydrogel model of idiopathic pulmonary fibrosis.Microcirculation (New York, N.Y. : 1994) · 2023Article
- Lymphatic microcirculation profile in the progression of hypertension in spontaneously hypertensive rats.Microcirculation (New York, N.Y. : 1994) · 2022Article
- A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?Frontiers in bioengineering and biotechnology · 2022Review
- Bioprinting on Live Tissue for Investigating Cancer Cell Dynamics.Tissue engineering. Part A · 2021Article
- A novel tissue culture model for evaluating the effect of aging on stem cell fate in adult microvascular networks.GeroScience · 2020Article
- Lymphatic-to-blood vessel transition in adult microvascular networks: A discovery made possible by a top-down approach to biomimetic model development.Microcirculation (New York, N.Y. : 1994) · 2020Article
- Bioreactor System to Perfuse Mesentery Microvascular Networks and Study Flow Effects During Angiogenesis.Tissue engineering. Part C, Methods · 2019Article
- Understanding angiogenesis during aging: opportunities for discoveries and new models.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Review
- Induction of microvascular network growth in the mouse mesentery.Microcirculation (New York, N.Y. : 1994) · 2018Article
- Endothelial Cell Phenotypes are Maintained During Angiogenesis in Cultured Microvascular Networks.Scientific reports · 2018Article
- A Novel ex vivo Mouse Mesometrium Culture Model for Investigating Angiogenesis in Microvascular Networks.Journal of vascular research · 2018Article
- Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model.Scientific reports · 2017Article
- An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks.Journal of visualized experiments : JoVE · 2017Article
- Aging is associated with impaired angiogenesis, but normal microvascular network structure, in the rat mesentery.American journal of physiology. Heart and circulatory physiology · 2017Article
- Laser Direct-Write Onto Live Tissues: A Novel Model for Studying Cancer Cell Migration.Journal of cellular physiology · 2016Article
- Lysophosphatidic acid does not cause blood/lymphatic vessel plasticity in the rat mesentery culture model.Physiological reports · 2016Article
- Printing cancer cells into intact microvascular networks: a model for investigating cancer cell dynamics during angiogenesis.Integrative biology : quantitative biosciences from nano to macro · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
New models of angiogenesis that mimic the complexity of real microvascular networks are needed. Recently, our laboratory demonstrated that cultured rat mesentery tissues contain viable microvascular networks and could be used to probe pericyte-endothelial cell interactions. The objective of this study was to demonstrate the efficacy of the rat mesentery culture model for anti-angiogenic drug testing by time-lapse quantification of network growth. Mesenteric windows were harvested from adult rats, secured in place with an insert, and cultured for 3 days according to 3 experimental groups: 1) 10% serum (angiogenesis control), 2) 10% serum + sunitinib (SU11248), and 3) 10% serum + bevacizumab. Labeling with FITC conjugated BSI-lectin on Day 0 and 3 identified endothelial cells along blood and lymphatic microvascular networks. Comparison between day 0 (before) and 3 (after) in networks stimulated by 10% serum demonstrated a dramatic increase in vascular density and capillary sprouting. Growing networks contained proliferating endothelial cells and NG2+ vascular pericytes. Media supplementation with sunitinib (SU11248) or bevacizumab both inhibited the network angiogenic responses. The comparison of the same networks before and after treatment enabled the identification of tissue specific responses. Our results establish, for the first time, the ability to evaluate an anti-angiogenic drug based on time-lapse imaging on an intact microvascular network in an ex vivo scenario.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.