ArticleJournal of visualized experiments : JoVE2015
Dorsal root ganglia neurons and differentiated adipose-derived stem cells: an in vitro co-culture model to study peripheral nerve regeneration.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Self-Assembling Peptide Hydrogels Support Stromal Vascular Fraction Viability to Promote In Vivo Nerve Regeneration.Advanced healthcare materials · 2026Article
- Golgi-mediated microtubule nucleation is associated with initiation of vertebrate peripheral neuron regeneration.iScience · 2025Article
- Acetylcholine muscarinic M2 receptor maintains human Schwann-like adipose-derived phenotype in the absence of differentiating medium.Cell death discovery · 2025Article
- In Vitro Models for Peripheral Nerve Regeneration.Advanced healthcare materials · 2024Review
- Identification of vagal afferent nerve endings in the mouse colon and their spatial relationship with enterochromaffin cells.Cell and tissue research · 2024Article
- Review
- Human platelet lysate as a potential clinical-translatable supplement to support the neurotrophic properties of human adipose-derived stem cells.Stem cell research & therapy · 2020Article
- Glial Cell-Axonal Growth Cone Interactions in Neurodevelopment and Regeneration.Frontiers in neuroscience · 2020Review
- GABA-B1 Receptor-Null Schwann Cells Exhibit Compromised In Vitro Myelination.Molecular neurobiology · 2019Article
- M2 receptors activation modulates cell growth, migration and differentiation of rat Schwann-like adipose-derived stem cells.Cell death discovery · 2019Article
- Allogenic Adipose Derived Stem Cells Transplantation Improved Sciatic Nerve Regeneration in Rats: Autologous Nerve Graft Model.Frontiers in pharmacology · 2018Article
- Polymeric scaffolds for three-dimensional culture of nerve cells: a model of peripheral nerve regeneration.MRS communications · 2017Article
- Bioactive Fraction ofFrontiers in cellular neuroscience · 2017Article
- Extracellular matrix from human umbilical cord-derived mesenchymal stem cells as a scaffold for peripheral nerve regeneration.Neural regeneration research · 2016Article
- A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia.BMC research notes · 2016Article
- Feasibility of Human Amniotic Fluid Derived Stem Cells in Alleviation of Neuropathic Pain in Chronic Constrictive Injury Nerve Model.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dorsal root ganglia (DRG) neurons, located in the intervertebral foramina of the spinal column, can be used to create an in vitro system facilitating the study of nerve regeneration and myelination. The glial cells of the peripheral nervous system, Schwann cells (SC), are key facilitators of these processes; it is therefore crucial that the interactions of these cellular components are studied together. Direct contact between DRG neurons and glial cells provides additional stimuli sensed by specific membrane receptors, further improving the neuronal response. SC release growth factors and proteins in the culture medium, which enhance neuron survival and stimulate neurite sprouting and extension. However, SC require long proliferation time to be used for tissue engineering applications and the sacrifice of an healthy nerve for their sourcing. Adipose-derived stem cells (ASC) differentiated into SC phenotype are a valid alternative to SC for the set-up of a co-culture model with DRG neurons to study nerve regeneration. The present work presents a detailed and reproducible step-by-step protocol to harvest both DRG neurons and ASC from adult rats; to differentiate ASC towards a SC phenotype; and combines the two cell types in a direct co-culture system to investigate the interplay between neurons and SC in the peripheral nervous system. This tool has great potential in the optimization of tissue-engineered constructs for peripheral nerve repair.
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