ArticlePloS one2015
Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73 citing papers in PubMed, 163 citations in OpenAlex.
- The FaDOF2-FaMYB4 transcriptional module regulates anthocyanin biosynthesis in strawberry fruit.Plant cell reports · 2026Article
- Identification and Expression Profile of the Chalcone Synthase (International journal of molecular sciences · 2026Article
- Molecular and Biochemical Impact of Selenium on the Acceleration of Ripening and Quality Changes in 'Camarosa' Strawberry Fruits.Plants (Basel, Switzerland) · 2026Article
- The role of RaDFR2 regulating flower color formation in Rhododendron agastum.BMC plant biology · 2026Article
- Transcriptome-based resistance analysis of susceptible and resistant tomato cultivars againstFrontiers in plant science · 2026Article
- Integrative transcriptomic and metabolomic analysis reveals the molecular basis of leaf variegation inFrontiers in plant science · 2026Article
- Unraveling the multifaceted roles of theForestry research · 2026Article
- Genome-Wide Analysis of the FNSII Gene Family and the Role of CitFNSII-1 in Flavonoid Synthesis in Citrus.Plants (Basel, Switzerland) · 2025Article
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- Genome-wide identification, characterization and expression analysis of the chalcone synthase gene family in Chinese cabbage.BMC genomics · 2025Article
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- Influence of Secondary Metabolites According to Maturation of Perilla (International journal of molecular sciences · 2024Article
- Review
- Effect of Origin, Seed Coat Color, and Maturity Group on Seed Isoflavones in Diverse Soybean Germplasm.Plants (Basel, Switzerland) · 2024Article
- Structural and Interactional Analysis of the Flavonoid Pathway Proteins: Chalcone Synthase, Chalcone Isomerase and Chalcone Isomerase-like Protein.International journal of molecular sciences · 2024Article
- Chalcone-Synthase-EncodingMolecules (Basel, Switzerland) · 2024Article
- Engineering a Plant Polyketide Synthase for the Biosynthesis of Methylated Flavonoids.Journal of agricultural and food chemistry · 2024Article
- HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd.PloS one · 2024Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chalcone synthase (CHS) catalyzes the first committed step in the flavonoid biosynthetic pathway. In this study, the cDNA (FhCHS1) encoding CHS from Freesia hybrida was successfully isolated and analyzed. Multiple sequence alignments showed that both the conserved CHS active site residues and CHS signature sequence were found in the deduced amino acid sequence of FhCHS1. Meanwhile, crystallographic analysis revealed that protein structure of FhCHS1 is highly similar to that of alfalfa CHS2, and the biochemical analysis results indicated that it has an enzymatic role in naringenin biosynthesis. Moreover, quantitative real-time PCR was performed to detect the transcript levels of FhCHS1 in flowers and different tissues, and patterns of FhCHS1 expression in flowers showed significant correlation to the accumulation patterns of anthocyanin during flower development. To further characterize the functionality of FhCHS1, its ectopic expression in Arabidopsis thaliana tt4 mutants and Petunia hybrida was performed. The results showed that overexpression of FhCHS1 in tt4 mutants fully restored the pigmentation phenotype of the seed coats, cotyledons and hypocotyls, while transgenic petunia expressing FhCHS1 showed flower color alteration from white to pink. In summary, these results suggest that FhCHS1 plays an essential role in the biosynthesis of flavonoid in Freesia hybrida and may be used to modify the components of flavonoids in other plants.
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