ArticlePLoS neglected tropical diseases2015
Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Pathogens of the Human Central Nervous System-the Major Protozoan Parasites and Helminths.Molecular neurobiology · 2026Review
- Humanization of CD47 enables development of functional human neutrophils via postirradiation remodeling of the bone marrow.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evasive mechanisms of human VSG and PfEMP1 antigens with link to Vaccine scenario: a review.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Review
- Ontogeny, functions and reprogramming of Kupffer cells upon infectious disease.Frontiers in immunology · 2023Review
- A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatileRSC chemical biology · 2022Article
- Neutrophils impose strong immune pressure against PfEMP1 variants implicated in cerebral malaria.EMBO reports · 2022Article
- Clinical Progression ofPathogens (Basel, Switzerland) · 2022Article
- Trypanosoma brucei triggers a broad immune response in the adipose tissue.PLoS pathogens · 2021Article
- The Macrophage Iron Signature in Health and Disease.International journal of molecular sciences · 2021Review
- A role for hepcidin in the anemia caused by Trypanosoma brucei infection.Haematologica · 2021Article
- Hepatocyte-derived IL-10 plays a crucial role in attenuating pathogenicity during the chronic phase of T. congolense infection.PLoS pathogens · 2020Article
- Protein expression-independent response of intensity-based pH-sensitive fluorophores in Escherichia coli.PloS one · 2020Article
- Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages.Journal of visualized experiments : JoVE · 2019Article
- The Complement System Is Essential for the Phagocytosis of Mesenchymal Stromal Cells by Monocytes.Frontiers in immunology · 2019Article
- Neutrophils enhance early Trypanosoma brucei infection onset.Scientific reports · 2018Article
- African Trypanosomiasis-Associated Anemia: The Contribution of the Interplay between Parasites and the Mononuclear Phagocyte System.Frontiers in immunology · 2018Review
- From the Cradle to the Grave: The Role of Macrophages in Erythropoiesis and Erythrophagocytosis.Frontiers in immunology · 2017Review
- Interferon Gamma in African Trypanosome Infections: Friends or Foes?Frontiers in immunology · 2017Review
- MIF-Mediated Hemodilution Promotes Pathogenic Anemia in Experimental African Trypanosomosis.PLoS pathogens · 2016Article
- Vesicles as Vehicles for Virulence.Trends in parasitology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular trypanosomes can cause a wide range of diseases and pathological complications in a broad range of mammalian hosts. One common feature of trypanosomosis is the occurrence of anemia, caused by an imbalance between erythropoiesis and red blood cell clearance of aging erythrocytes. In murine models for T. brucei trypanosomosis, anemia is marked by a very sudden non-hemolytic loss of RBCs during the first-peak parasitemia control, followed by a short recovery phase and the subsequent gradual occurrence of an ever-increasing level of anemia. Using a newly developed quantitative pHrodo based in vitro erythrophagocytosis assay, combined with FACS-based ex vivo and in vivo results, we show that activated liver monocytic cells and neutrophils as well as activated splenic macrophages are the main cells involved in the occurrence of the early-stage acute anemia. In addition, we show that trypanosomosis itself leads to a rapid alteration of RBC membrane stability, priming the cells for accelerated phagocytosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.