ArticlePLoS neglected tropical diseases2015
Circulation of Crimean-Congo Hemorrhagic Fever Virus in the former Yugoslav Republic of Macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Seroprevalence of Crimean-Congo hemorrhagic fever in humans in the World Health Organization European region: A systematic review.PLoS neglected tropical diseases · 2020Pooled it
- Crimean-Congo Hemorrhagic Fever Infection in the Small Ruminant Population in N. Macedonia: A Seroepidemiological Study as a Step Towards Better Understanding of the CCHF Epidemiology in the Country.Pathogens (Basel, Switzerland) · 2026Article
- Crimean-Congo hemorrhagic fever: An emerging threat in Europe.Virologica Sinica · 2026Review
- Serological insights of Crimean-Congo haemorrhagic fever virus in podolic cattle bred in Italy.Frontiers in cellular and infection microbiology · 2026Article
- Serological evidence of tick-borne Crimean-Congo haemorrhagic fever and Dugbe orthonairovirus infections in cattle in Kwara State in northern Nigeria indicate independent endemics.PLoS neglected tropical diseases · 2024Article
- First Broad-Range Serological Survey of Crimean-Congo Hemorrhagic Fever among Hungarian Livestock.Viruses · 2024Article
- Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans.Parasites & vectors · 2024Review
- Crimean-Congo haemorrhagic fever virus in ticks, domestic, and wild animals.Frontiers in veterinary science · 2024Review
- Cases of Crimean-Congo haemorrhagic fever in North Macedonia, July to August 2023.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Article
- Mediterranean spotted fever-like illness caused byEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2022Article
- Development of anti-Crimean-Congo hemorrhagic fever virus Gc and NP-specific ELISA for detection of antibodies in domestic animal sera.Frontiers in veterinary science · 2022Article
- Seroprevalence and Risk Factors of Crimean-Congo Hemorrhagic Fever in Cattle of Smallholder Farmers in Central Malawi.Pathogens (Basel, Switzerland) · 2021Article
- Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa.Veterinary medicine and science · 2021Article
- Article
- Deciphering Antibody Responses to Orthonairoviruses in Ruminants.Microorganisms · 2021Article
- First Serological Evidence of Crimean-Congo Hemorrhagic Fever Virus and Rift Valley Fever Virus in Ruminants in Tunisia.Pathogens (Basel, Switzerland) · 2021Article
- Crimean-Congo hemorrhagic fever virus antibody prevalence in Mauritanian livestock (cattle, goats, sheep and camels) is stratified by the animal's age.PLoS neglected tropical diseases · 2021Article
- Article
- Crimean-Congo Hemorrhagic Fever Virus (CCHFV): A Silent but Widespread Threat.Current tropical medicine reports · 2021Review
- Sheep and Cattle Are Not Susceptible to Experimental Inoculation with Hazara Orthonairovirus, a Tick-Borne Arbovirus Closely Related to CCHFV.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
16 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere are only few assays available for the detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-specific antibodies in animals, and data about diagnostic sensitivity and specificity are incompletely documented for most of these tests. This is unfortunate since CCHFV antibodies in animals can be used as indicator for virus circulation in a geographic area and therewith potential risk of human exposure. This paper therefore reports on a novel ELISA for the detection of CCHFV-specific antibodies in cattle and on its application for testing ruminant sera from the Former Yugoslav Republic of Macedonia. PRINCIPAL
findingsA highly sensitive and specific ELISA was developed to detect CCHFV-specific IgG antibodies in cattle. The assay was validated by using 503 negative serum samples from a country where CCHFV has never been detected until now, and by using 54 positive serum samples. The positive sera were verified by using two commercially available assays (for testing human serum) which we have adapted for use in animals. The sensitivity of the novel ELISA was 98% and its specificity 99%. The presence of Hyalomma ticks was demonstrated in the Former Yugoslav Republic of Macedonia and depending on the region antibody prevalence rates up to 80% were detected in the cattle population.
conclusionThis article describes a fully validated, highly sensitive and specific ELISA for the detection of CCHFV-specific IgG antibodies in cattle. Using this assay, CCHFV-specific antibodies were detected for the first time in cattle in the Former Yugoslav Republic of Macedonia, giving evidence for an active circulation of this virus in the country. Supporting this conclusion, the occurrence of the main vector of CCHFV was demonstrated in the present work for the first time in Former Yugoslav Republic of Macedonia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.