ArticleJournal of visualized experiments : JoVE2015
In situ Ca2+ imaging of the enteric nervous system.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 24 citations in OpenAlex.
- Imaging the enteric nervous system.Frontiers in neuroanatomy · 2025Review
- Highly neurogenic glia from human and mouse myenteric ganglia generate functional neurons following culture and transplantation into the gut.Cell reports · 2024Article
- In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window.Nature communications · 2024Article
- Calcium image analysis in the moving gut.Neurogastroenterology and motility · 2023Article
- Stimulator of interferon genes (STING) expression in the enteric nervous system and contributions of glial STING in disease.Neurogastroenterology and motility · 2023Article
- Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.Bioengineering & translational medicine · 2023Article
- Impact of cryopreservation on viability, gene expression and function of enteric nervous system derived neurospheres.Frontiers in cell and developmental biology · 2023Article
- Functional Intraregional and Interregional Heterogeneity between Myenteric Glial Cells of the Colon and Duodenum in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Enteric glial biology, intercellular signalling and roles in gastrointestinal disease.Nature reviews. Gastroenterology & hepatology · 2021Review
- Histamine-dependent interactions between mast cells, glia, and neurons are altered following early-life adversity in mice and humans.American journal of physiology. Gastrointestinal and liver physiology · 2020Article
- Enlightening the frontiers of neurogastroenterology through optogenetics.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Effects of Serotonin and Slow-Release 5-Hydroxytryptophan on Gastrointestinal Motility in a Mouse Model of Depression.Gastroenterology · 2019Article
- DRG-Derived Neural Progenitors Differentiate into Functional Enteric Neurons Following Transplantation in the Postnatal Colon.Cell transplantation · 2019Article
- A Modular Assembly of Spinal Cord-Like Tissue Allows Targeted Tissue Repair in the Transected Spinal Cord.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Opportunities and Challenges for Single-Unit Recordings from Enteric Neurons in Awake Animals.Micromachines · 2018Review
- Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse.Journal of visualized experiments : JoVE · 2018Article
- Induction of alpha-synuclein pathology in the enteric nervous system of the rat and non-human primate results in gastrointestinal dysmotility and transient CNS pathology.Neurobiology of disease · 2018Article
- The acute inhibition of enteric glial metabolism with fluoroacetate alters calcium signaling, hemichannel function, and the expression of key proteins.Journal of neurophysiology · 2017Article
- Agonist-evoked CaCellular and molecular gastroenterology and hepatology · 2015Article
- PasteurizedGut microbesArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Reflex behaviors of the intestine are controlled by the enteric nervous system (ENS). The ENS is an integrative network of neurons and glia in two ganglionated plexuses housed in the gut wall. Enteric neurons and enteric glia are the only cell types within the enteric ganglia. The activity of enteric neurons and glia is responsible for coordinating intestinal functions. This protocol describes methods for observing the activity of neurons and glia within the intact ENS by imaging intracellular calcium (Ca(2+)) transients with fluorescent indicator dyes. Our technical discussion focuses on methods for Ca(2+) imaging in whole-mount preparations of the myenteric plexus from the rodent bowel. Bulk loading of ENS whole-mounts with a high-affinity Ca(2+) indicator such as Fluo-4 permits measurements of Ca(2+) responses in individual neurons or glial cells. These responses can be evoked repeatedly and reliably, which permits quantitative studies using pharmacological tools. Ca(2+) responses in cells of the ENS are recorded using a fluorescence microscope equipped with a cooled charge-coupled device (CCD) camera. Fluorescence measurements obtained using Ca(2+) imaging in whole-mount preparations offer a straightforward means of characterizing the mechanisms and potential functional consequences of Ca(2+) responses in enteric neurons and glial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.