ArticleJournal of visualized experiments : JoVE2015
Preparation and respirometric assessment of mitochondria isolated from skeletal muscle tissue obtained by percutaneous needle biopsy.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Resistance exercise training and in vitro skeletal muscle oxidative capacity in older adults.Physiological reports · 2016Trial
- Muscle mitochondria, function, mass, and quality of life in prostate cancer during androgen deprivation therapy.Nature communications · 2026Article
- Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation.BMC research notes · 2023Article
- Article
- Cardiomyopathic mutations in essential light chain reveal mechanisms regulating the super relaxed state of myosin.The Journal of general physiology · 2021Article
- Mitochondrial and Glycolytic Capacity of Peripheral Blood Mononuclear Cells Isolated From Diverse Poultry Genetic Lines: Optimization and Assessment.Frontiers in veterinary science · 2021Article
- Mitochondrial electron transport chain: Oxidative phosphorylation, oxidant production, and methods of measurement.Redox biology · 2020Review
- Isolation of mitochondria from cells and tissues.Methods in cell biology · 2020Article
- A Low Iron Diet Protects from Steatohepatitis in a Mouse Model.Nutrients · 2019Article
- Endometrium and endometriosis tissue mitochondrial energy metabolism in a nonhuman primate model.Reproductive biology and endocrinology : RB&E · 2019Article
- Mitochondria-targeted Probes for Imaging Protein Sulfenylation.Scientific reports · 2018Article
- Skeletal muscle mitochondrial mass is linked to lipid and metabolic profile in individuals with spinal cord injury.European journal of applied physiology · 2017Article
- Mitochondrial mass and activity as a function of body composition in individuals with spinal cord injury.Physiological reports · 2017Article
- Blood-Based Bioenergetic Profiling Reflects Differences in Brain Bioenergetics and Metabolism.Oxidative medicine and cellular longevity · 2017Article
- Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health.Redox biology · 2016Article
- Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells.Journal of visualized experiments : JoVE · 2016Article
- Skeletal muscle mitochondrial health and spinal cord injury.World journal of orthopedics · 2016Review
- Skeletal Muscle Mitochondrial Content, Oxidative Capacity, and Mfn2 Expression Are Reduced in Older Patients With Heart Failure and Preserved Ejection Fraction and Are Related to Exercise Intolerance.JACC. Heart failure · 2016Article
- Relationships between mitochondrial content and bioenergetics with obesity, body composition and fat distribution in healthy older adults.BMC obesity · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining samples of human Vastus lateralis, isolating mitochondria from minimal amounts of skeletal muscle tissue, and plate based respirometric profiling using an extracellular flux (XF) analyzer. Comparison of respirometric profiles obtained using 1.0, 2.5 and 5.0 μg of mitochondria indicate that 1.0 μg is sufficient to measure respiration and that 5.0 μg provides most consistent results based on comparison of standard errors. Western blot analysis of isolated mitochondria for mitochondrial marker COX IV and non-mitochondrial tissue marker GAPDH indicate that there is limited non-mitochondrial contamination using this protocol. The ability to study mitochondrial respirometry in as little as 20 mg of muscle tissue allows users to utilize individual biopsies for multiple study endpoints in clinical research projects.
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