ArticleThe Journal of cell biology1989
Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 150 citations in OpenAlex.
- Review
- The N-Terminal Part ofInternational journal of molecular sciences · 2023Article
- Coordination of Embryogenesis by the Centrosome in Drosophila melanogaster.Results and problems in cell differentiation · 2019Review
- Article
- Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster.BMC biology · 2015Article
- Dynamin regulates metaphase furrow formation and plasma membrane compartmentalization in the syncytial Drosophila embryo.Biology open · 2015Article
- Newly Characterized Region of CP190 Associates with Microtubules and Mediates Proper Spindle Morphology in Drosophila Stem Cells.PloS one · 2015Article
- Why are bacteria different from eukaryotes?BMC biology · 2013Article
- The elegans of spindle assembly.Cellular and molecular life sciences : CMLS · 2010Review
- On and around microtubules: an overview.Molecular biotechnology · 2009Review
- Article
- Wac: a new Augmin subunit required for chromosome alignment but not for acentrosomal microtubule assembly in female meiosis.The Journal of cell biology · 2009Article
- A microtubule interactome: complexes with roles in cell cycle and mitosis.PLoS biology · 2008Article
- The functional domain grouping of microtubule associated proteins.Communicative & integrative biology · 2008Article
- Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells.The Journal of cell biology · 2002Article
- The drosophila protein asp is involved in microtubule organization during spindle formation and cytokinesis.The Journal of cell biology · 2001Article
- The TACC domain identifies a family of centrosomal proteins that can interact with microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Lava lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularization.The Journal of cell biology · 2000Article
- Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster.The Journal of cell biology · 2000Article
- Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo. More than 50 Drosophila embryo proteins bind to microtubule affinity columns. To begin to characterize these proteins, we have generated individual mouse polyclonal antibodies that specifically recognize 24 of them. As judged by immunofluorescence, some of the antigens localize to the mitotic spindle in the early Drosophila embryo, while others are present in centrosomes, kinetochores, subsets of microtubules, or a combination of these structures. Since 20 of the 24 antibodies stain microtubule structures, it is likely that most of the proteins that bind to our columns are associated with microtubules in vivo. Very few MAPS seem to be identically localized in the cell, indicating that the microtubule cytoskeleton is remarkably complex.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.