ArticlePloS one2015
Spindle assembly checkpoint acquisition at the mid-blastula transition.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Optochemical elucidation of a critical role of the incomplete spindle assembly checkpoint in zebrafish development.Communications biology · 2026Article
- Haploidy-linked cell proliferation defects limit larval growth in zebrafish.Open biology · 2024Article
- Chromosome Division in Early Embryos-Is Everything under Control? And Is the Cell Size Important?International journal of molecular sciences · 2024Review
- Collective effects of cell cleavage dynamics.Frontiers in cell and developmental biology · 2024Review
- Early onset of APC/C activity renders SAC inefficient in mouse embryos.Frontiers in cell and developmental biology · 2024Article
- Spindle assembly checkpoint insensitivity allows meiosis-II despite chromosomal defects in aged eggs.EMBO reports · 2023Article
- BUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses.Current biology : CB · 2023Article
- Review
- Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns.Scientific reports · 2021Article
- How Many Is Enough? - Challenges of Multinucleated Cell Division in Malaria Parasites.Frontiers in cellular and infection microbiology · 2021Review
- The Spindle Assembly Checkpoint Functions during Early Development in Non-Chordate Embryos.Cells · 2020Article
- The mechanisms and clinical application of mosaicism in preimplantation embryos.Journal of assisted reproduction and genetics · 2020Review
- A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation.Nature communications · 2019Article
- Spindle assembly checkpoint strength is linked to cell fate in the Caenorhabditis elegans embryo.Molecular biology of the cell · 2018Article
- UBASH3B-mediated silencing of the mitotic checkpoint: Therapeutic perspectives in cancer.Molecular & cellular oncology · 2018Article
- Chromosome biorientation and APC activity remain uncoupled in oocytes with reduced volume.The Journal of cell biology · 2017Article
- "Wait anaphase" signals are not confined to the mitotic spindle.Molecular biology of the cell · 2017Article
- Cell Size Determines the Strength of the Spindle Assembly Checkpoint during Embryonic Development.Developmental cell · 2016Article
- Direct Unequal Cleavages: Embryo Developmental Competence, Genetic Constitution and Clinical Outcome.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The spindle assembly checkpoint (SAC) maintains the fidelity of chromosome segregation during mitosis. Nonpathogenic cells lacking the SAC are typically only found in cleavage stage metazoan embryos, which do not acquire functional checkpoints until the mid-blastula transition (MBT). It is unclear how proper SAC function is acquired at the MBT, though several models exist. First, SAC acquisition could rely on transcriptional activity, which increases dramatically at the MBT. Embryogenesis prior to the MBT relies primarily on maternally loaded transcripts, and if SAC signaling components are not maternally supplied, the SAC would depend on zygotic transcription at the MBT. Second, checkpoint acquisition could depend on the Chk1 kinase, which is activated at the MBT to elongate cell cycles and is required for the SAC in somatic cells. Third, SAC function could depend on a threshold nuclear to cytoplasmic (N:C) ratio, which increases during pre-MBT cleavage cycles and dictates several MBT events like zygotic transcription and cell cycle remodeling. Finally, the SAC could by regulated by a timer mechanism that coincides with other MBT events but is independent of them. Using zebrafish embryos we show that SAC acquisition at the MBT is independent of zygotic transcription, indicating that the checkpoint program is maternally supplied. Additionally, by precociously lengthening cleavage cycles with exogenous Chk1 activity, we show that cell cycle lengthening and Chk1 activity are not sufficient for SAC acquisition. Furthermore, we find that SAC acquisition can be uncoupled from the N:C ratio. Together, our findings indicate that SAC acquisition is regulated by a maternally programmed developmental timer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.