ArticleWorld journal of gastroenterology2015
Biological effects of low-dose-rate irradiation of pancreatic carcinoma cells in vitro using 125I seeds.
Article in World journal of gastroenterology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Histopathological evaluation of local effects of radioactive iodine seeds in axillary lymph nodes in clinically node-positive breast cancer treated with neoadjuvant systemic therapy.Breast (Edinburgh, Scotland) · 2026Article
- Tolerance limit of external beam radiotherapy combined with low-dose rate brachytherapy in normal rabbit tissue.Journal of radiation research · 2023Article
- The efficacy and safety ofQuantitative imaging in medicine and surgery · 2023Article
- Article
- Microdosimetric and radiobiological effects of gold nanoparticles at therapeutic radiation energies.International journal of radiation biology · 2023Article
- Cytotoxicity of Metal-Based Nanoparticles: From Mechanisms and Methods of Evaluation to Pathological Manifestations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Palliative local treatment of bone metastases by 125I seed brachytherapy under DynaCT guidance: single-center experience.Diagnostic and interventional radiology (Ankara, Turkey) · 2021Article
- To explore the curative effect of CT-guided Iodine-125 radioactive seed implantation in the treatment of stage Ⅳprimary hepatocellular carcinoma.Journal of interventional medicine · 2021Article
- Lentinan Combined with (125)I Brachytherapy for Recurrent Ovarian Cancer.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Eukaryotic Translation Initiation Factor 3 Subunit B Is a Promoter in the Development and Progression of Pancreatic Cancer.Frontiers in oncology · 2021Article
- Article
- Iodine-125 seed implantation for residual hepatocellular carcinoma or cholangiocellular carcinoma in challenging locations after transcatheter arterial chemoembolization: Initial experience and findings.Journal of contemporary brachytherapy · 2020Article
- Iodine‑125 seed radiation induces ROS‑mediated apoptosis, autophagy and paraptosis in human esophageal squamous cell carcinoma cells.Oncology reports · 2020Article
- Evaluation of Iodine-125 Interstitial Brachytherapy Using Micro-Positron Emission Tomography/Computed Tomography with 18F-Fluorodeoxyglucose in Hepatocellular Carcinoma HepG2 Xenografts.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- The efficacy ofInternational journal of clinical and experimental pathology · 2019Article
- Preliminary application of 3D-printed coplanar template for iodine-125 seed implantation therapy in patients with advanced pancreatic cancer.World journal of gastroenterology · 2018Article
- Numb/Notch signaling pathway modulation enhances human pancreatic cancer cell radiosensitivity.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Combination of 125I brachytherapy and chemotherapy for unresectable recurrent breast cancer: A retrospective control study.Medicine · 2016Article
- Continuous Low-dose-rate Irradiation of Iodine-125 Seeds Inhibiting Perineural Invasion in Pancreatic Cancer.Chinese medical journal · 2016Article
- Clinical efficacy of computed tomography-guided iodine-125 seed implantation therapy in patients with advanced spinal metastatic tumors.OncoTargets and therapy · 2016Article
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Authors and funding
10 authors.
Funding
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Abstract
aimTo determine the mechanism of the radiation-induced biological effects of 125I seeds on pancreatic carcinoma cells in vitro.
methodsSW1990 and PANC-1 pancreatic cancer cell lines were cultured in DMEM in a suitable environment. Gray's model of iodine-125 (125I) seed irradiation was used. In vitro, exponential phase SW1990, and PANC-1 cells were exposed to 0, 2, 4, 6, and 8 Gy using 125I radioactive seeds, with an initial dose rate of 12.13 cGy/h. A clonogenic survival experiment was performed to observe the ability of the cells to maintain their clonogenic capacity and to form colonies. Cell-cycle and apoptosis analyses were conducted to detect the apoptosis percentage in the SW1990 and PANC-1 cells. DNA synthesis was measured via a tritiated thymidine (3H-TdR) incorporation experiment. After continuous low-dose-rate irradiation with 125I radioactive seeds, the survival fractions at 2 Gy (SF2), percentage apoptosis, and cell cycle phases of the SW1990 and PANC-1 pancreatic cancer cell lines were calculated and compared.
resultsThe survival fractions of the PANC-1 and SW1990 cells irradiated with 125I seeds decreased exponentially as the dose increased. No significant difference in SF2 was observed between SW1990 and PANC-1 cells (0.766±0.063 vs 0.729±0.045, P<0.05). The 125I seeds induced a higher percentage of apoptosis than that observed in the control in both the SW1990 and PANC-1 cells. The rate of apoptosis increased with increasing radiation dosage. The percentage of apoptosis was slightly higher in the SW1990 cells than in the PANC-1 cells. Dose-dependent G2/M cell-cycle arrest was observed after 125I seed irradiation, with a peak value at 6 Gy. As the dose increased, the percentage of G2/M cell cycle arrest increased in both cell lines, whereas the rate of DNA incorporation decreased. In the 3H-TdR incorporation experiment, the dosimetry results of both the SW1990 and PANC-1 cells decreased as the radiation dose increased, with a minimum at 6 Gy. There were no significant differences in the dosimetry results of the two cell lines when they were exposed to the same dose of radiation.
conclusionThe pancreatic cancer cell-killing effects induced by 125I radioactive seeds mainly occurred via apoptosis and G2/M cell cycle arrest.
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