ArticleG3 (Bethesda, Md.)2015
Rapid and Efficient Identification of Caenorhabditis elegans Legacy Mutations Using Hawaiian SNP-Based Mapping and Whole-Genome Sequencing.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Vitamin BNature communications · 2026Article
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Vitamin BResearch square · 2025Article
- Identification of a new allele ofmicroPublication biology · 2025Article
- Article
- Article
- Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.G3 (Bethesda, Md.) · 2021Article
- The molecular underpinnings of fertility: Genetic approaches inAdvanced genetics (Hoboken, N.J.) · 2021Article
- Harnessing the power of genetics: fast forward genetics in Caenorhabditis elegans.Molecular genetics and genomics : MGG · 2021Review
- PhenoMIP: High-Throughput Phenotyping of DiverseG3 (Bethesda, Md.) · 2020Article
- Article
- ThemicroPublication biology · 2020Article
- Identification of Suppressors ofG3 (Bethesda, Md.) · 2020Article
- Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans.BMC biology · 2018Article
- New Role for an Old Protein: An Educational Primer for Use with "The Identification of a Novel Mutant Allele ofGenetics · 2018Review
- MIP-MAP: High-Throughput Mapping ofGenetics · 2017Article
- Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans.Current protocols in molecular biology · 2017Article
- Evaluating alignment and variant-calling software for mutation identification in C. elegans by whole-genome sequencing.PloS one · 2017Article
- Article
- Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans.Genetics · 2016Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The production of viable embryos requires the coordination of many cellular processes, including protein synthesis, cytoskeletal reorganization, establishment of polarity, cell migration, cell division, and in Caenorhabditis elegans, eggshell formation. Defects in any of these processes can lead to embryonic lethality. We examined six temperature-sensitive mutants as well as one nonconditional mutant that were previously identified in genetic screens as either embryonic lethal (maternal-effect or zygotic lethal) or eggshell defective. The responsible molecular lesion for each had never been determined. After confirmation of temperature sensitivity and lethality, we performed whole-genome sequencing using a single-nucleotide polymorphism mapping strategy to pinpoint the molecular lesions. Gene candidates were confirmed by RNA interference phenocopy and/or complementation tests and one mutant was further validated by CRISPR (Clustered Regularly Interspaced Short Palidromic Repeats)/Cas9 gene editing. This approach identified new alleles of several genes that had only been previously studied by RNA interference depletion. Our identification of temperature-sensitive alleles for all of these essential genes provides an extremely useful tool for further investigation for the C. elegans community, such as the ability to address mutant phenotypes at various developmental stages and the ability to carry out suppressor/enhancer screens to identify other genes that function in a specific cellular process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.