ArticleJournal of experimental botany2015
Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes.Nucleus (Austin, Tex.) · 2022Article
- Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.Plant physiology · 2022Review
- The role of ATXR6 expression in modulating genome stability and transposable element repression inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story.International journal of molecular sciences · 2021Article
- A live imaging system to analyze spatiotemporal dynamics of RNA polymerase II modification in Arabidopsis thaliana.Communications biology · 2021Article
- Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes.International journal of molecular sciences · 2021Article
- Prospects and limitations of expansion microscopy in chromatin ultrastructure determination.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- Tidying-up the plant nuclear space: domains, functions, and dynamics.Journal of experimental botany · 2020Review
- Nucleolus-associated chromatin domains are maintained under heat stress, despite nucleolar reorganization in Arabidopsis thaliana.Journal of plant research · 2020Article
- CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing.Plant biotechnology journal · 2020Review
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Ribosomal RNA genes shape chromatin domains associating with the nucleolus.Nucleus (Austin, Tex.) · 2019Review
- Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.Nucleus (Austin, Tex.) · 2019Review
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- Advances in Imaging Plant Cell Dynamics.Plant physiology · 2018Review
- Tackling Plant Meiosis: From Model Research to Crop Improvement.Frontiers in plant science · 2018Review
- Conserved redox-dependent DNA binding of ROXY glutaredoxins with TGA transcription factors.Plant direct · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA polymerase II (RNAPII) is responsible for the transcription of most eukaryotic protein-coding genes. Analysing the topological distribution and quantification of RNAPII can contribute to understanding its function in interphase nuclei. Previously it was shown that RNAPII molecules in plant nuclei form reticulate structures within euchromatin of differentiated Arabidopsis thaliana nuclei rather than being organized in distinct 'transcription factories' as observed in mammalian nuclei. Immunosignal intensity measurements based on specific antibody labelling in maximum intensity projections of image stacks acquired by structured illumination microscopy (SIM) suggested a relative proportional increase of RNAPII in endopolyploid plant nuclei. Here, photoactivated localization microscopy (PALM) was applied to determine the absolute number and distribution of active and inactive RNAPII molecules in differentiated A. thaliana nuclei. The proportional increase of RNAPII during endopolyploidization is confirmed, but it is also shown that PALM measurements are more reliable than those based on SIM in terms of quantification. The single molecule localization results show that, although RNAPII molecules are globally dispersed within plant euchromatin, they also aggregate within smaller distances as described for mammalian transcription factories.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.