ArticleJournal of clinical microbiology2015
Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 2 of them syntheses that pooled it.
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Who cites it
46 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Diagnostic Accuracy of Multiplex NAAT/PCR and Culture AgainstPathogens (Basel, Switzerland) · 2025Pooled it
- Diagnostic accuracy of multiplex nucleic acid amplification tests forPathogens and global health · 2023Pooled it
- Multicentre Evaluation of Cepheid XpertMicroorganisms · 2026Article
- Effect of Diagnostic Test Type on Detection of Salmonella Infections - Foodborne Diseases Active Surveillance Network, 2004-2024.MMWR. Morbidity and mortality weekly report · 2026Article
- Repeat multiplex PCR gastrointestinal panel testing within 14 days yields minimal additional diagnostic information: a multicenter cohort study.Infection control and hospital epidemiology · 2026Article
- Identification of a new Salmonella serovar - Salmonella Weitmar (8:z41:1,5).GMS infectious diseases · 2026Article
- Multiplex Polymerase Chain Reaction Panels for Gastrointestinal Infections: Current Evidence, Regulatory Hurdles, and the Way Forward.Open forum infectious diseases · 2025Article
- Acute infectious gastroenteritis in childhood: the role of rapid multiplex molecular syndromic panels in diagnosis and clinical management.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2025Review
- Performance evaluation of the Biosynex AMPLIQUICK Fecal Bacteriology PCR kit.BMC microbiology · 2025Article
- The Effect of Mitomycin C on Induction of Shiga Toxin Production in Clinical STEC Isolates.Toxins · 2025Article
- Identification of a new Salmonella serovar - Salmonella Scharmann (8:z41:1,5).GMS infectious diseases · 2025Article
- Evaluation of non-invasive diagnostic tools for diarrhea: a systematic review of point-of-care tests and biomarkers.Annals of medicine and surgery (2012) · 2024Review
- Evaluation of the BioFire Gastrointestinal Panel to Detect Diarrheal Pathogens in Pediatric Patients.Diagnostics (Basel, Switzerland) · 2021Article
- Application of a Multiplex Polymerase Chain Reaction Test for Diagnosing Bacterial Enteritis in Children in a Real-Life Clinical Setting.Children (Basel, Switzerland) · 2021Article
- Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation ofFrontiers in cellular and infection microbiology · 2021Article
- Panels and Syndromic Testing in Clinical Microbiology.Clinics in laboratory medicine · 2020Review
- Performance Evaluation of the Novodiag Bacterial GE+ Multiplex PCR Assay.Journal of clinical microbiology · 2020Article
- Efficacy of stool multiplex polymerase chain reaction assay in adult patients with acute infectious diarrhea.World journal of clinical cases · 2020Article
- Do changes in STEC diagnostics mislead interpretation of disease surveillance data in Switzerland? Time trends in positivity, 2007 to 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
- Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diarrhea due to enteric bacterial pathogens causes significant morbidity and mortality in the United States and worldwide. However, bacterial pathogens may be infrequently identified. Currently, culture and enzyme immunoassays (EIAs) are the primary methods used by clinical laboratories to detect enteric bacterial pathogens. We conducted a multicenter evaluation of the BD Max enteric bacterial panel (EBP) PCR assay in comparison to culture for the detection of Salmonella spp., Shigella spp., Campylobacter jejuni, and Campylobacter coli and an EIA for Shiga toxins 1 and 2. A total of 4,242 preserved or unpreserved stool specimens, including 3,457 specimens collected prospectively and 785 frozen, retrospective samples, were evaluated. Compared to culture or EIA, the positive percent agreement (PPA) and negative percent agreement (NPA) values for the BD Max EBP assay for all specimens combined were as follows: 97.1% and 99.2% for Salmonella spp., 99.1% and 99.7% for Shigella spp., 97.2% and 98.4% for C. jejuni and C. coli, and 97.4% and 99.3% for Shiga toxins, respectively. Discrepant results for prospective samples were resolved with alternate PCR assays and bidirectional sequencing of amplicons. Following discrepant analysis, PPA and NPA values were as follows: 97.3% and 99.8% for Salmonella spp., 99.2% and 100% for Shigella spp., 97.5% and 99.0% for C. jejuni and C. coli, and 100% and 99.7% for Shiga toxins, respectively. No differences in detection were observed for samples preserved in Cary-Blair medium and unpreserved samples. In this large, multicenter study, the BD Max EBP assay showed superior sensitivity compared to conventional methods and excellent specificity for the detection of enteric bacterial pathogens in stool specimens.
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