ArticleThe Journal of reproduction and development2015
A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos.
Article in The Journal of reproduction and development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Expanded iOn switch toolkit enables flexible clonal labeling and dynamic imaging in model and non-model animals.Communications biology · 2026Article
- Optimization of piggyBac transposon-mediated gene transfer method in common marmoset embryos.PloS one · 2023Article
- Efficient generation of epigenetic disease model mice by epigenome editing using the piggyBac transposon system.Epigenetics & chromatin · 2022Article
- Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon.Scientific reports · 2016Article
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5 authors.
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Abstract
Transgenic mice are important tools for genetic analysis. A current prominent method for producing transgenic mice involves pronuclear microinjection into 1-cell embryos. However, the total transgenic efficiency obtained using this method is less than 10%. Here, we demonstrate that highly efficient transgenesis in mice can be achieved by cytoplasmic microinjection using a hyperactive piggyBac system. In embryos in which hyPBase mRNA and pPB-CAG-TagRFP DNA were co-injected into the cytoplasm, TagRFP fluorescence was observed after the 2-cell stage; when 30 ng/µl pPB-CAG-TagRFP DNA and 30 ng/µl hyPBase mRNA were co-injected, 94.4% of blastocysts were TagRFP positive. Furthermore, a high concentration of hyPBase mRNA resulted in creation of mosaic embryos in which the TagRFP signals partially disappeared. However, suitable concentrations of injected DNA and hyPBase mRNA produced embryos in which almost all blastomeres were TagRFP positive. Thus, the hyperactive piggyBac transposon system is an easy-to-implement and highly effective method that can contribute to production of transgenic mice.
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