ArticleJournal of proteome research2015
Host-pathogen interaction profiling using self-assembling human protein arrays.
Article in Journal of proteome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Dual-species proteomics and targeted intervention of animal-pathogen interactions.Journal of advanced research · 2025Article
- Monitoring host-pathogen interactions using chemical proteomics.RSC chemical biology · 2024Review
- From prediction to function: Current practices and challenges towards the functional characterization of type III effectors.Frontiers in microbiology · 2023Review
- Host-microbiome protein-protein interactions capture disease-relevant pathways.Genome biology · 2022Article
- The role of pathogens in diabetes pathogenesis and the potential of immunoproteomics as a diagnostic and prognostic tool.Frontiers in microbiology · 2022Review
- Developments and Applications of Functional Protein Microarrays.Molecular & cellular proteomics : MCP · 2020Review
- The specific seroreactivity to ∆Np73 isoforms shows higher diagnostic ability in colorectal cancer patients than the canonical p73 protein.Scientific reports · 2019Article
- Experimental Analysis of Viral-Host Interactions.Frontiers in physiology · 2019Review
- Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites.Malaria journal · 2018Article
- Legionella effector AnkX interacts with host nuclear protein PLEKHN1.BMC microbiology · 2018Article
- Insights from the sequence similarity of Zika virus proteins with the Human nerve proteins.Bioinformation · 2018Article
- Elucidation of host-pathogen protein-protein interactions to uncover mechanisms of host cell rewiring.Current opinion in microbiology · 2017Review
- Proteomics Tracing the Footsteps of Infectious Disease.Molecular & cellular proteomics : MCP · 2017Review
- Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.Current protocols in cell biology · 2017Article
- The Toolbox for Uncovering the Functions ofFrontiers in cellular and infection microbiology · 2017Review
- Technologies for Proteome-Wide Discovery of Extracellular Host-Pathogen Interactions.Journal of immunology research · 2017Review
- Multiplexed Nucleic Acid Programmable Protein Arrays.Theranostics · 2017Article
- Beyond Paralogs: The Multiple Layers of Redundancy in Bacterial Pathogenesis.Frontiers in cellular and infection microbiology · 2017Review
- Type II Secretion Is Necessary for Optimal Association of the Legionella-Containing Vacuole with Macrophage Rab1B but Enhances Intracellular Replication Mainly by Rab1B-Independent Mechanisms.Infection and immunity · 2016Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Host-pathogen protein interactions are fundamental to every microbial infection, yet their identification has remained challenging due to the lack of simple detection tools that avoid abundance biases while providing an open format for experimental modifications. Here, we applied the Nucleic Acid-Programmable Protein Array and a HaloTag-Halo ligand detection system to determine the interaction network of Legionella pneumophila effectors (SidM and LidA) with 10 000 unique human proteins. We identified known targets of these L. pneumophila proteins and potentially novel interaction candidates. In addition, we applied our Click chemistry-based NAPPA platform to identify the substrates for SidM, an effector with an adenylyl transferase domain that catalyzes AMPylation (adenylylation), the covalent addition of adenosine monophosphate (AMP). We confirmed a subset of the novel SidM and LidA targets in independent in vitro pull-down and in vivo cell-based assays, and provided further insight into how these effectors may discriminate between different host Rab GTPases. Our method circumvents the purification of thousands of human and pathogen proteins, and does not require antibodies against or prelabeling of query proteins. This system is amenable to high-throughput analysis of effectors from a wide variety of human pathogens that may bind to and/or post-translationally modify targets within the human proteome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.