ArticlePlant physiology2015
The micro-RNA72c-APETALA2-1 node as a key regulator of the common bean-Rhizobium etli nitrogen fixation symbiosis.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 118 citations in OpenAlex.
- RNA regulation in plants.Science China. Life sciences · 2026Review
- PGPR-Mediated Plant Immunity: From Microbial Recognition to Epigenetic Priming.Plants (Basel, Switzerland) · 2026Review
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Hormone-modulated transcription factors orchestrating the root nodule symbiosis.Frontiers in plant science · 2026Review
- Exogenous Application of ENOD40 and CEP1 Peptides Boosts Symbiotic Signaling Gene Expression and Productivity in Common Bean.Plants (Basel, Switzerland) · 2025Article
- Non-conserved microRNAs and their roles in plants: the case for legumes.Plant biology (Stuttgart, Germany) · 2025Review
- Transposon-Associated Small RNAs Involved in Plant Defense in Poplar.Plants (Basel, Switzerland) · 2025Article
- Identification of microRNAs involved in the Phosphate starvation response in Oil Palm (Elaeis guineensis Jacq.).Molecular biology reports · 2023Article
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- A nitrogen fixing symbiosis-specific pathway required for legume flowering.Science advances · 2023Article
- SPL12 RegulatesPlants (Basel, Switzerland) · 2022Article
- Integrated Degradome and Srna Sequencing Revealed miRNA-mRNA Regulatory Networks between the Phloem and Developing Xylem of Poplar.International journal of molecular sciences · 2022Article
- The miR156b-GmSPL9d module modulates nodulation by targeting multiple core nodulation genes in soybean.The New phytologist · 2022Article
- Integrated mRNA and microRNA expression analysis of root response to phosphate deficiency inFrontiers in plant science · 2022Article
- Development of plant systemic resistance by beneficial rhizobacteria: Recognition, initiation, elicitation and regulation.Frontiers in plant science · 2022Review
- Identification and Functional Prediction of Poplar Root circRNAs Involved in Treatment With Different Forms of Nitrogen.Frontiers in plant science · 2022Article
- Genome-Wide Analysis of Long Non-coding RNAs Involved in Nodule Senescence inFrontiers in plant science · 2022Article
- Integrated Analysis of Physiological, mRNA Sequencing, and miRNA Sequencing Data Reveals a Specific Mechanism for the Response to Continuous Cropping Obstacles inFrontiers in plant science · 2022Article
- Plant Transcription Factors Involved in Drought and Associated Stresses.International journal of molecular sciences · 2021Review
- miRNAomic Approach to Plant Nitrogen Starvation.International journal of genomics · 2021Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Micro-RNAs are recognized as important posttranscriptional regulators in plants. The relevance of micro-RNAs as regulators of the legume-rhizobia nitrogen-fixing symbiosis is emerging. The objective of this work was to functionally characterize the role of micro-RNA172 (miR172) and its conserved target APETALA2 (AP2) transcription factor in the common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis. Our expression analysis revealed that mature miR172c increased upon rhizobial infection and continued increasing during nodule development, reaching its maximum in mature nodules and decaying in senescent nodules. The expression of AP2-1 target showed a negative correlation with miR172c expression. A drastic decrease in miR172c and high AP2-1 mRNA levels were observed in ineffective nodules. Phenotypic analysis of composite bean plants with transgenic roots overexpressing miR172c or a mutated AP2-1 insensitive to miR172c cleavage demonstrated the pivotal regulatory role of the miR172 node in the common bean-rhizobia symbiosis. Increased miR172 resulted in improved root growth, increased rhizobial infection, increased expression of early nodulation and autoregulation of nodulation genes, and improved nodulation and nitrogen fixation. In addition, these plants showed decreased sensitivity to nitrate inhibition of nodulation. Through transcriptome analysis, we identified 114 common bean genes that coexpressed with AP2-1 and proposed these as being targets for transcriptional activation by AP2-1. Several of these genes are related to nodule senescence, and we propose that they have to be silenced, through miR172c-induced AP2-1 cleavage, in active mature nodules. Our work sets the basis for exploring the miR172-mediated improvement of symbiotic nitrogen fixation in common bean, the most important grain legume for human consumption.
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