ArticlePlant physiology2015
The Xanthomonas campestris type III effector XopJ proteolytically degrades proteasome subunit RPT6.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Rethinking arsenal: non-TAL effectors drive Xanthomonas virulence in Brassica.Plant physiology · 2026Article
- The Recognition of YopJ Family Effectors Depends on ZAR1/JIM2 Immune Complex in Nicotiana benthamiana.Molecular plant pathology · 2026Article
- Tomato Chlorotic Virus Minor Coat Protein Interferes With the Host Proteasome Function by Competitive Disruption of SlPAD1-SlPA4 Interaction to Enhance Viral Infection.Molecular plant pathology · 2025Article
- DOMAIN OF UNKNOWN FUNCTION581-9 negatively regulates SnRK1 kinase activity.Plant physiology · 2024Article
- A Pipeline to Monitor Proteasome Homeostasis in Plants.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component.The EMBO journal · 2022Article
- Pathogens pulling the strings: Effectors manipulating salicylic acid and phenylpropanoid biosynthesis in plants.Molecular plant pathology · 2021Review
- Host manipulation by bacterial type III and type IV secretion system effector proteases.Cellular microbiology · 2021Review
- Bacterial effectors mimicking ubiquitin-proteasome pathway tweak plant immunity.Microbiological research · 2021Review
- What the Wild Things Do: Mechanisms of Plant Host Manipulation by Bacterial Type III-Secreted Effector Proteins.Microorganisms · 2021Review
- Secrete or perish: The role of secretion systems inComputational and structural biotechnology journal · 2021Review
- The 26S Proteasome Regulatory Subunit GmPSMD Promotes Resistance toFrontiers in plant science · 2021Article
- Natural Variation in Virulence ofThe plant pathology journal · 2020Article
- The Role of Proteases in the Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2019Review
- Bacteria Exploit Autophagy for Proteasome Degradation and Enhanced Virulence in Plants.The Plant cell · 2018Article
- The Xanthomonas euvesicatoria type III effector XopAU is an active protein kinase that manipulates plant MAP kinase signaling.PLoS pathogens · 2018Article
- Ubiquitin Proteasome Activity Measurement in Total Plant Extracts.Bio-protocol · 2017Article
- The type III effector AvrXccB in Xanthomonas campestris pv. campestris targets putative methyltransferases and suppresses innate immunity in Arabidopsis.Molecular plant pathology · 2017Article
- A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response.Scientific reports · 2017Article
- YopJ Family Effectors Promote Bacterial Infection through a Unique Acetyltransferase Activity.Microbiology and molecular biology reviews : MMBR · 2016Review
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many animal and plant pathogenic bacteria inject type III effector (T3E) proteins into their eukaryotic host cells to suppress immunity. The Yersinia outer protein J (YopJ) family of T3Es is a widely distributed family of effector proteins found in both animal and plant pathogens, and its members are highly diversified in virulence functions. Some members have been shown to possess acetyltransferase activity; however, whether this is a general feature of YopJ family T3Es is currently unknown. The T3E Xanthomonas outer protein J (XopJ), a YopJ family effector from the plant pathogen Xanthomonas campestris pv vesicatoria, interacts with the proteasomal subunit Regulatory Particle AAA-ATPase6 (RPT6) in planta to suppress proteasome activity, resulting in the inhibition of salicylic acid-related immune responses. Here, we show that XopJ has protease activity to specifically degrade RPT6, leading to reduced proteasome activity in the cytoplasm as well as in the nucleus. Proteolytic degradation of RPT6 was dependent on the localization of XopJ to the plasma membrane as well as on its catalytic triad. Mutation of the Walker B motif of RPT6 prevented XopJ-mediated degradation of the protein but not XopJ interaction. This indicates that the interaction of RPT6 with XopJ is dependent on the ATP-binding activity of RPT6, but proteolytic cleavage additionally requires its ATPase activity. Inhibition of the proteasome impairs the proteasomal turnover of Nonexpressor of Pathogenesis-Related1 (NPR1), the master regulator of salicylic acid responses, leading to the accumulation of ubiquitinated NPR1, which likely interferes with the full induction of NPR1 target genes. Our results show that YopJ family T3Es are not only highly diversified in virulence function but also appear to possess different biochemical activities.
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