ArticleInternational journal of molecular sciences2015
Chloroplast-expressed MSI-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Antimicrobial Peptides: Classification, Mechanism, and Application in Plant Disease Resistance.Probiotics and antimicrobial proteins · 2025Review
- Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts.BMC biotechnology · 2023Article
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean.BMC genetics · 2020Article
- Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials.Scientific reports · 2019Article
- Antimicrobial Peptides: Recent Insights on Biotechnological Interventions and Future Perspectives.Protein and peptide letters · 2019Review
- Recent achievements obtained by chloroplast transformation.Plant methods · 2017Review
- Review
- Chloroplast genomes: diversity, evolution, and applications in genetic engineering.Genome biology · 2016Review
- Discovery of the molecular mechanisms of the novel chalcone-basedSpringerPlus · 2016Article
- Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco.International journal of molecular sciences · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast.
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