ArticleJournal of the American Chemical Society2015
What controls the hybridization thermodynamics of spherical nucleic acids?
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Spherical Nucleic Acids: Turning Synthetic Advances and Fundamental Discovery into Translational Breakthroughs in Chemistry, Materials Development, Biology, and Medicine.Accounts of chemical research · 2026Article
- Engineering spherical nucleic acids for precision cancer therapy: design strategies and medical applications.Theranostics · 2026Review
- POSA for fast, amplified and multiplexed protein imaging.Nature communications · 2025Article
- Improved Catalytic Activity of Spherical Nucleic Acid Enzymes by Hybridization Chain Reaction and Its Application for Sensitive Analysis of Aflatoxin B1.Sensors (Basel, Switzerland) · 2024Article
- Exploring the Structural Diversity of DNA Bottlebrush Polymers Using an Oligonucleotide Macromonomer Approach.Macromolecules · 2022Article
- Hairpin-like siRNA-Based Spherical Nucleic Acids.Journal of the American Chemical Society · 2022Article
- Synthesis of an Azide- and Tetrazine-Functionalized [60]Fullerene and Its Controlled Decoration with Biomolecules.ACS omega · 2022Article
- Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core.Bioconjugate chemistry · 2021Article
- Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Nanoparticle-based computing architecture for nanoparticle neural networks.Science advances · 2020Article
- Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK.Diagnostics (Basel, Switzerland) · 2020Article
- Engineering DNA-Functionalized Nanostructures to Bind Nucleic Acid Targets Heteromultivalently with Enhanced Avidity.Journal of the American Chemical Society · 2020Article
- The effector mechanism of siRNA spherical nucleic acids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Expanding the materials space of DNA via organic-phase ring-opening metathesis polymerization.Chem · 2019Article
- The Role of Structural Enthalpy in Spherical Nucleic Acid Hybridization.Journal of the American Chemical Society · 2018Article
- The competing effects of core rigidity and linker flexibility in the nanoassembly of trivalent small molecule-DNA hybrids (SMDHNanoscale · 2017Article
- Nanoparticle-Programmed Surface for Drug Release and Cell Regulation via Reversible Hybridization Reaction.ACS applied materials & interfaces · 2017Article
- Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Relationships between Poly(ethylene glycol) Modifications on RNA-Spherical Nucleic Acid Conjugates and Cellular Uptake and Circulation Time.Bioconjugate chemistry · 2016Article
- Design Considerations for RNA Spherical Nucleic Acids (SNAs).Bioconjugate chemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
The hybridization of free oligonucleotides to densely packed, oriented arrays of DNA modifying the surfaces of spherical nucleic acid (SNA)-gold nanoparticle conjugates occurs with negative cooperativity; i.e., each binding event destabilizes subsequent binding events. DNA hybridization is thus an ever-changing function of the number of strands already hybridized to the particle. Thermodynamic quantification of this behavior reveals a 3 orders of magnitude decrease in the binding constant for the capture of a free oligonucleotide by an SNA conjugate as the fraction of pre-hybridized strands increases from 0 to ∼30%. Increasing the number of pre-hybridized strands imparts an increasing enthalpic penalty to hybridization that makes binding more difficult, while simultaneously decreasing the entropic penalty to hybridization, which makes binding more favorable. Hybridization of free DNA to an SNA is thus governed by both an electrostatic barrier as the SNA accumulates charge with additional binding events and an effect consistent with allostery, where hybridization at certain sites on an SNA modify the binding affinity at a distal site through conformational changes to the remaining single strands. Leveraging these insights allows for the design of conjugates that hybridize free strands with significantly higher efficiencies, some of which approach 100%.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.