ArticlePloS one2015
A novel video tracking method to evaluate the effect of influenza infection and antiviral treatment on ferret activity.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Updated A(H1N1)pdm09 influenza virus ferret infection model permits refined antiviral assessment using aerosol inhalation challenge.PLoS pathogens · 2026Article
- Eleven quick tips to unlock the power of in vivo data science.PLoS computational biology · 2025Article
- Key considerations to improve the normalization, interpretation and reproducibility of morbidity data in mammalian models of viral disease.Disease models & mechanisms · 2024Article
- Article
- Ferrets as Models for Viral Respiratory Disease.Comparative medicine · 2023Review
- The impact of stress and anesthesia on animal models of infectious disease.Frontiers in veterinary science · 2023Review
- Assessing the fitness of a dual-antiviral drug resistant human influenza virus in the ferret model.Communications biology · 2022Article
- Article
- Bayesian Multi-Targets Strategy to TrackInsects · 2022Article
- Evaluating the fitness of PA/I38T-substituted influenza A viruses with reduced baloxavir susceptibility in a competitive mixtures ferret model.PLoS pathogens · 2021Article
- Utilising animal models to evaluate oseltamivir efficacy against influenza A and B viruses with reduced in vitro susceptibility.PLoS pathogens · 2020Article
- Baloxavir treatment of ferrets infected with influenza A(H1N1)pdm09 virus reduces onward transmission.PLoS pathogens · 2020Article
- Validation of alternative behavioral observation methods in young broiler chickens.Poultry science · 2019Article
- Untargeted metabolomics analysis of the upper respiratory tract of ferrets following influenza A virus infection and oseltamivir treatment.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- SwarmSight: Measuring the temporal progression of animal group activity levels from natural-scene and laboratory videos.Behavior research methods · 2017Article
- Complexities in Ferret Influenza Virus Pathogenesis and Transmission Models.Microbiology and molecular biology reviews : MMBR · 2016Review
- Debate Regarding Oseltamivir Use for Seasonal and Pandemic Influenza.Emerging infectious diseases · 2016Article
- Using the Ferret as an Animal Model for Investigating Influenza Antiviral Effectiveness.Frontiers in microbiology · 2016Review
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Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ferrets are the preferred animal model to assess influenza virus infection, virulence and transmission as they display similar clinical symptoms and pathogenesis to those of humans. Measures of disease severity in the ferret include weight loss, temperature rise, sneezing, viral shedding and reduced activity. To date, the only available method for activity measurement has been the assignment of an arbitrary score by a 'blind' observer based on pre-defined responsiveness scale. This manual scoring method is subjective and can be prone to bias. In this study, we described a novel video-tracking methodology for determining activity changes in a ferret model of influenza infection. This method eliminates the various limitations of manual scoring, which include the need for a sole 'blind' observer and the requirement to recognise the 'normal' activity of ferrets in order to assign relative activity scores. In ferrets infected with an A(H1N1)pdm09 virus, video-tracking was more sensitive than manual scoring in detecting ferret activity changes. Using this video-tracking method, oseltamivir treatment was found to ameliorate the effect of influenza infection on activity in ferret. Oseltamivir treatment of animals was associated with an improvement in clinical symptoms, including reduced inflammatory responses in the upper respiratory tract, lower body weight loss and a smaller rise in body temperature, despite there being no significant reduction in viral shedding. In summary, this novel video-tracking is an easy-to-use, objective and sensitive methodology for measuring ferret activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.