ArticlePLoS pathogens2015
Interaction between the type III effector VopO and GEF-H1 activates the RhoA-ROCK pathway.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- ThemSphere · 2025Article
- Role of the TPR family protein VPA1365 in regulating type III secretion system 2 and virulence inApplied and environmental microbiology · 2025Article
- VopX, a novelmBio · 2025Article
- PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells.Stem cell research & therapy · 2024Article
- Contribution of the Type III Secretion System (T3SS2) ofMicroorganisms · 2024Article
- Article
- Reducing GEF-H1 Expression Inhibits Renal Cyst Formation, Inflammation, and Fibrosis via RhoA Signaling in Nephronophthisis.International journal of molecular sciences · 2023Article
- Vibrio parahaemolyticus Epidemiology and Pathogenesis: Novel Insights on an Emerging Foodborne Pathogen.Advances in experimental medicine and biology · 2023Article
- Article
- Identification of a Family ofmSphere · 2021Article
- Adaptations ofFrontiers in microbiology · 2021Review
- Bartonella effector protein C mediates actin stress fiber formation via recruitment of GEF-H1 to the plasma membrane.PLoS pathogens · 2021Article
- Bartonella type IV secretion effector BepC induces stress fiber formation through activation of GEF-H1.PLoS pathogens · 2021Article
- Article
- Vibrio variations on a type three theme.Current opinion in microbiology · 2019Review
- Article
- Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA.Journal of cell science · 2017Article
- Effect of shRNA targeted against RhoA on proliferation and migration of human colonic cancer cells.International journal of clinical and experimental pathology · 2015Article
- Regulation and functions of the RhoA regulatory guanine nucleotide exchange factor GEF-H1.Small GTPasesReview
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio parahaemolyticus is an important pathogen that causes food-borne gastroenteritis in humans. The type III secretion system encoded on chromosome 2 (T3SS2) plays a critical role in the enterotoxic activity of V. parahaemolyticus. Previous studies have demonstrated that T3SS2 induces actin stress fibers in various epithelial cell lines during infection. This stress fiber formation is strongly related to pathogenicity, but the mechanisms that underlie T3SS2-dependent actin stress fiber formation and the main effector have not been elucidated. In this study, we identified VopO as a critical T3SS2 effector protein that activates the RhoA-ROCK pathway, which is an essential pathway for the induction of the T3SS2-dependent stress fiber formation. We also determined that GEF-H1, a RhoA guanine nucleotide exchange factor (GEF), directly binds VopO and is necessary for T3SS2-dependent stress fiber formation. The GEF-H1-binding activity of VopO via an alpha helix region correlated well with its stress fiber-inducing capacity. Furthermore, we showed that VopO is involved in the T3SS2-dependent disruption of the epithelial barrier. Thus, VopO hijacks the RhoA-ROCK pathway in a different manner compared with previously reported bacterial toxins and effectors that modulate the Rho GTPase signaling pathway.
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