ArticleConnective tissue research2015
Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy.
Article in Connective tissue research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Articular Cartilage-From Basic Science Structural Imaging to Non-Invasive Clinical Quantitative Molecular Functional Information for AI Classification and Prediction.International journal of molecular sciences · 2023Review
- Label-free, multi-parametric assessments of cell metabolism and matrix remodeling within human and early-stage murine osteoarthritic articular cartilage.Communications biology · 2023Article
- Review
- Characterization of collagen response to bone fracture healing using polarization-SHG.Scientific reports · 2022Article
- Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy.Osteoarthritis and cartilage · 2021Article
- Glucose Availability Affects Extracellular Matrix Synthesis During ChondrogenesisTissue engineering. Part A · 2021Article
- Spatial frequency metrics for analysis of microscopic images of musculoskeletal tissues.Connective tissue research · 2021Article
- Murine Metatarsus Bone and Joint Collagen-I Fiber Morphologies and Networks Studied With SHG Multiphoton Imaging.Frontiers in bioengineering and biotechnology · 2021Article
- Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.BME frontiers · 2021Review
- Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy.Journal of the Royal Society, Interface · 2019Article
- Non-linear optical microscopy of cartilage canals in the distal femur of young pigs may reveal the cause of articular osteochondrosis.BMC veterinary research · 2017Article
- Wavelength-Dependent Second Harmonic Generation Circular Dichroism for Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer Based on Intrinsic Chirality Differences.The journal of physical chemistry. B · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe collagen structure throughout the patella has not been thoroughly investigated by 3D imaging, where the majority of the existing data come from histological cross sections. It is important to have a better understanding of the architecture in normal tissues, where this could then be applied to imaging of diseased states.
methodsTo address this shortcoming, we investigated the combined use of collagen-specific Second-Harmonic Generation (SHG) imaging and measurement of bulk optical properties to characterize collagen fiber orientations of the histologically defined zones of bovine articular cartilage. Forward and backward SHG intensities of sections from superficial, middle and deep zones were collected as a function of depth and analyzed by Monte Carlo simulations to extract the SHG creation direction, which is related to the fibrillar assembly.
resultsOur results revealed differences in SHG forward-backward response between the three zones, where these are consistent with a previously developed model of SHG emission. Some of the findings are consistent with that from other modalities; however, SHG analysis showed the middle zone had the most organized fibril assembly. While not distinct, we also report bulk optical property values for these different zones within the patella.
conclusionsCollectively, these results provide quantitative measurements of structural changes at both the fiber and fibril assembly of the different cartilage zones and reveals structural information not possible by other microscope modalities. This can provide quantitative insight to the collagen fiber network in normal cartilage, which may ultimately be developed as a biomarker for osteoarthritis.
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Registered trials
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