ArticleMolecular metabolism2015
Seipin oligomers can interact directly with AGPAT2 and lipin 1, physically scaffolding critical regulators of adipogenesis.
Article in Molecular metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 90 citations in OpenAlex.
- Tripartite ER-mitochondria-lipid droplets contact sites control adipocyte metabolic flexibility.The EMBO journal · 2026Article
- Adipogenin promotes the development of lipid droplets by binding a dodecameric seipin complex.Science (New York, N.Y.) · 2025Article
- Targeting Seipin to Alleviate Hepatic Steatosis in Zebrafish (Danio Rerio).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Functional study of Phaeodactylum tricornutum Seipin highlights specificities of lipid droplets biogenesis in diatoms.The New phytologist · 2025Article
- The Role of theInternational journal of molecular sciences · 2025Review
- Article
- Targeting lipid droplets and lipid droplet-associated proteins: a new perspective on natural compounds against metabolic diseases.Chinese medicine · 2024Review
- A unifying mechanism for seipin-mediated lipid droplet formation.FEBS letters · 2024Review
- Concept of lipid droplet biogenesis.European journal of cell biology · 2023Review
- Structure and function of lipid droplet assembly complexes.Current opinion in structural biology · 2023Review
- Seipin-still a mysterious protein?Frontiers in cell and developmental biology · 2023Review
- Lipid Droplet-a New Target in Ischemic Heart Disease.Journal of cardiovascular translational research · 2022Review
- Role of Seipin in Human Diseases and Experimental Animal Models.Biomolecules · 2022Review
- Seipin collaborates with the ER membrane to control the sites of lipid droplet formation.Current opinion in cell biology · 2022Review
- BSCL2/Seipin deficiency in hearts causes cardiac energy deficit and dysfunction via inducing excessive lipid catabolism.Clinical and translational medicine · 2022Article
- Seipin Deficiency as a Model of Severe Adipocyte Dysfunction: Lessons from Rodent Models and Teaching for Human Disease.International journal of molecular sciences · 2022Review
- Seipin: harvesting fat and keeping adipocytes healthy.Trends in cell biology · 2021Review
- Celia's Encephalopathy (Journal of clinical medicine · 2021Review
- Severe insulin resistance syndromes.The Journal of clinical investigation · 2021Review
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveDisruption of the genes encoding either seipin or 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2) causes severe congenital generalized lipodystrophy (CGL) in humans. However, the function of seipin in adipogenesis remains poorly defined. We demonstrated recently that seipin can bind the key adipogenic phosphatidic acid (PA) phosphatase lipin 1 and that seipin forms stable dodecamers. As AGPAT2 generates PA, the substrate for lipin 1, we investigated whether seipin might bind both enzymes of this lipid biosynthetic pathway, which is required for adipogenesis to occur.
methodsWe employed co-immunoprecipitation and immunofluorescence methods to determine whether seipin can interact with AGPAT2 and the consequences of this in developing adipocytes. Atomic force microscopy was used to determine whether these interactions involved direct association of the proteins and to define the molecular architecture of these complexes.
resultsOur data reveal that seipin can bind AGPAT2 during adipogenesis and that stabilizing this interaction during adipogenesis can increase the nuclear accumulation of PPARγ. Both AGPAT2 and lipin 1 can directly associate with seipin dodecamers, and a single seipin complex can simultaneously bind both AGPAT2 and lipin with a defined orientation.
conclusionsOur study provides the first direct molecular link between seipin and AGPAT2, two proteins whose disruption causes CGL. Moreover, it provides the first example of an interaction between seipin and another protein that causally influences a key aspect of adipogenesis. Together our data suggest that the critical role of seipin in adipogenesis may involve its capacity to juxtapose important regulators of this process in a multi-protein complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.