ArticlePeerJ2015
Insight into Shiga toxin genes encoded by Escherichia coli O157 from whole genome sequencing.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 62 citations in OpenAlex.
- Pathogenome and Plasmid-Borne Antimicrobial Resistance Phenotypes in a Multidrug-Resistant O111:H8 Shiga Toxin-ProducingbioRxiv : the preprint server for biology · 2026Article
- Algorithm and Software to Type Stx Operons Accurately from Assembled Genomic Sequence.Microorganisms · 2026Article
- Enumeration and Genomic Confirmation of Viable Shiga-Toxin-ProducingPathogens (Basel, Switzerland) · 2026Article
- Mind the gap! TypingMicrobial genomics · 2026Article
- Predicting clinical outcome ofMicrobial genomics · 2025Article
- Shiga toxin-producingFrontiers in microbiology · 2025Article
- Genotypic analysis of Shiga toxin-producingJournal of medical microbiology · 2024Article
- Genomic analysis of an outbreak of Shiga toxin-producingMicrobial genomics · 2024Article
- A Comprehensive Review on Shiga Toxin Subtypes and Their Niche-Related Distribution Characteristics in Shiga-Toxin-ProducingMicroorganisms · 2024Review
- Detection ofFoods (Basel, Switzerland) · 2024Article
- Pathogenomes and virulence profiles of representative big six non-O157 serogroup Shiga toxin-producingFrontiers in microbiology · 2024Article
- Prevalence of Shiga Toxin-ProducingAnimals : an open access journal from MDPI · 2023Article
- Analysis ofMicrobial genomics · 2023Article
- Phylogenetic relationship and virulence composition ofFrontiers in microbiology · 2023Article
- Outbreak of STEC O157:H7 linked to a milk pasteurisation failure at a dairy farm in England, 2019.Epidemiology and infection · 2022Article
- Characterization of Atypical Shiga Toxin Gene Sequences and Description of Stx2j, a New Subtype.Journal of clinical microbiology · 2022Article
- Epidemiology and genomic analysis of Shiga toxin-producingJournal of medical microbiology · 2021Article
- Epidemiological investigations identified an outbreak of Shiga toxin-producingEpidemiology and infection · 2021Article
- Phylogenetic structure of Shiga toxin-producingMicrobial genomics · 2021Article
- Inconsistent PCR detection of Shiga toxin-producing Escherichia coli: Insights from whole genome sequence analyses.PloS one · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of Shiga toxin-producing Escherichia coli (STEC) to cause severe illness in humans is determined by multiple host factors and bacterial characteristics, including Shiga toxin (Stx) subtype. Given the link between Stx2a subtype and disease severity, we sought to identify the stx subtypes present in whole genome sequences (WGS) of 444 isolates of STEC O157. Difficulties in assembling the stx genes in some strains were overcome by using two complementary bioinformatics methods: mapping and de novo assembly. We compared the WGS analysis with the results obtained using a PCR approach and investigated the diversity within and between the subtypes. All strains of STEC O157 in this study had stx1a, stx2a or stx2c or a combination of these three genes. There was over 99% (442/444) concordance between PCR and WGS. When common source strains were excluded, 236/349 strains of STEC O157 had multiple copies of different Stx subtypes and 54 had multiple copies of the same Stx subtype. Of those strains harbouring multiple copies of the same Stx subtype, 33 had variants between the alleles while 21 had identical copies. Strains harbouring Stx2a only were most commonly found to have multiple alleles of the same subtype (42%). Both the PCR and WGS approach to stx subtyping provided a good level of sensitivity and specificity. In addition, the WGS data also showed there were a significant proportion of strains harbouring multiple alleles of the same Stx subtype associated with clinical disease in England.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.