ArticleThe Journal of biological chemistry2015
Obstructor A organizes matrix assembly at the apical cell surface to promote enzymatic cuticle maturation in Drosophila.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
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- Zona pellucida proteins Piopio and Dumpy are essential for the apical extracellular matrix assembly and survival of Locusta migratoria.Pest management science · 2026Article
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- Distinct cellular and molecular mechanisms contribute to the specificity of the two Drosophila melanogaster chitin synthases in chitin deposition.PLoS genetics · 2025Article
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- Peritrophin-like Genes Are Associated with Delousing Drug Response and Sensitivity in the Sea LouseInternational journal of molecular sciences · 2022Article
- Improving Polysaccharide-Based Chitin/Chitosan-Aerogel Materials by Learning from Genetics and Molecular Biology.Materials (Basel, Switzerland) · 2022Review
- The spider cuticle: a remarkable material toolbox for functional diversity.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2021Article
- The Effect of the Hypertrophy Virus (MdSGHV) on the Ultrastructure of the Salivary Glands of Musca domestica (Diptera: Muscidae).Journal of insect science (Online) · 2021Article
- The Broad Transcription Factor Links Hormonal Signaling, Gene Expression, and Cellular Morphogenesis Events DuringGenetics · 2020Article
- Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator.Insect science · 2020Article
- Glycosylhydrolase genes control respiratory tubes sizes and airway stability.Scientific reports · 2020Article
- Cuticular Structure Proteomics in the Pea AphidJournal of proteome research · 2020Article
- Insect Cuticular Chitin Contributes to Form and Function.Current pharmaceutical design · 2020Article
- Genome-wide identification of chitin-binding proteins and characterization of BmCBP1 in the silkworm, Bombyx mori.Insect science · 2019Article
- The putative C-type lectin Schlaff ensures epidermal barrier compactness in Drosophila.Scientific reports · 2019Article
- A comprehensive omics analysis and functional survey of cuticular proteins in the brown planthopper.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Group I chitin deacetylases are essential for higher order organization of chitin fibers in beetle cuticle.The Journal of biological chemistry · 2018Article
- An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.Nature communications · 2018Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assembly and maturation of the apical extracellular matrix (aECM) is crucial for protecting organisms, but underlying molecular mechanisms remain poorly understood. Epidermal cells secrete proteins and enzymes that assemble at the apical cell surface to provide epithelial integrity and stability during developmental growth and upon tissue damage. We analyzed molecular mechanisms of aECM assembly and identified the conserved chitin-binding protein Obst-A (Obstructor A) as an essential regulator. We show in Drosophila that Obst-A is required to coordinate protein and chitin matrix packaging at the apical cell surface during development. Secreted by epidermal cells, the Obst-A protein is specifically enriched in the apical assembly zone where matrix components are packaged into their highly ordered architecture. In obst-A null mutant larvae, the assembly zone is strongly diminished, resulting in severe disturbance of matrix scaffold organization and impaired aECM integrity. Furthermore, enzymes that support aECM stability are mislocalized. As a biological consequence, cuticle architecture, integrity, and function are disturbed in obst-A mutants, finally resulting in immediate lethality upon wounding. Our studies identify a new core organizing center, the assembly zone that controls aECM assembly at the apical cell surface. We propose a genetically conserved molecular mechanism by which Obst-A forms a matrix scaffold to coordinate trafficking and localization of proteins and enzymes in the newly deposited aECM. This mechanism is essential for maturation and stabilization of the aECM in a growing and remodeling epithelial tissue as an outermost barrier.
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