ArticleChembiochem : a European journal of chemical biology2015
Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1.
Article in Chembiochem : a European journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Cell-free protein synthesis and vesicle systems for programmable therapeutic manufacturing and delivery.Journal of biological engineering · 2025Review
- Engineering cell-free systems by chemoproteomic-assisted phenotypic screening.RSC chemical biology · 2024Article
- A cell-free strategy for host-specific profiling of intracellular antibiotic sensitivity and resistance.npj antimicrobials and resistance · 2023Article
- The Use of Cell-free Protein Synthesis to Push the Boundaries of Synthetic Biology.Biotechnology and bioprocess engineering : BBE · 2023Review
- Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Biochemistry of Aminoacyl tRNA Synthetase and tRNAs and Their Engineering for Cell-Free and Synthetic Cell Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts.Frontiers in bioengineering and biotechnology · 2021Article
- Article
- Methodologies for preparation of prokaryotic extracts for cell-free expression systems.Synthetic and systems biotechnology · 2020Review
- Cell-free systems for accelerating glycoprotein expression and biomanufacturing.Journal of industrial microbiology & biotechnology · 2020Review
- Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits.Nature communications · 2020Article
- Cell-free gene expression: an expanded repertoire of applications.Nature reviews. Genetics · 2020Review
- Strategies for in vitro engineering of the translation machinery.Nucleic acids research · 2020Review
- Review
- Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems.Frontiers in bioengineering and biotechnology · 2020Review
- Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli.Cell chemical biology · 2019Article
- Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems.Synthetic and systems biotechnology · 2019Article
- Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.Applied microbiology and biotechnology · 2019Review
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9 authors.
Funding
Abstract
Site-specific incorporation of non-standard amino acids (NSAAs) into proteins opens the way to novel biological insights and applications in biotechnology. Here, we describe the development of a high yielding cell-free protein synthesis (CFPS) platform for NSAA incorporation from crude extracts of genomically recoded Escherichia coli lacking release factor 1. We used genome engineering to construct synthetic organisms that, upon cell lysis, lead to improved extract performance. We targeted five potential negative effectors to be disabled: the nuclease genes rna, rnb, csdA, mazF, and endA. Using our most productive extract from strain MCJ.559 (csdA(-) endA(-)), we synthesized 550±40 μg mL(-1) of modified superfolder green fluorescent protein containing p-acetyl-L-phenylalanine. This yield was increased to ∼1300 μg mL(-1) when using a semicontinuous method. Our work has implications for using whole genome editing for CFPS strain development, expanding the chemistry of biological systems, and cell-free synthetic biology.
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