ArticleScientific reports2015
Genome engineering and direct cloning of antibiotic gene clusters via phage ϕBT1 integrase-mediated site-specific recombination in Streptomyces.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 78 citations in OpenAlex.
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- An Improved Transformation-Associated Recombination Cloning Approach for Direct Capturing of Natural Product Biosynthetic Gene Clusters.Microbial biotechnology · 2024Article
- Variable orthogonality of serine integrase interactions within the ϕC31 family.Scientific reports · 2024Article
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- Variable orthogonality of RDF - large serine integrase interactions within the ϕC31 family.bioRxiv : the preprint server for biology · 2024Article
- An efficient method for targeted cloning of large DNA fragments from Streptomyces.Applied microbiology and biotechnology · 2023Article
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- Genetically engineered bacterium: Principles, practices, and prospects.Frontiers in microbiology · 2022Review
- Improving the Yield and Quality of Daptomycin inFrontiers in microbiology · 2022Article
- Molecular mechanism of mureidomycin biosynthesis activated by introduction of an exogenous regulatory gene ssaA into Streptomyces roseosporus.Science China. Life sciences · 2021Article
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- Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2021Review
- Recent Advances in Silent Gene Cluster Activation inFrontiers in bioengineering and biotechnology · 2021Review
- Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2020Review
- Cloning and Overexpression of theFrontiers in microbiology · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110.Microbial cell factories · 2019Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting of a specialized recombinase and its target sites. In this study, a novel strategy based on phage ϕBT1 integrase-mediated site-specific recombination was developed, and used for simultaneous Streptomyces genome engineering and cloning of antibiotic gene clusters. This method has been proved successful for the cloning of actinorhodin gene cluster from Streptomyces coelicolor M145, napsamycin gene cluster and daptomycin gene cluster from Streptomyces roseosporus NRRL 15998 at a frequency higher than 80%. Furthermore, the system could be used to increase the titer of antibiotics as we demonstrated with actinorhodin and daptomycin, and it will be broadly applicable in many Streptomyces.
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