ArticleFEMS yeast research2015
A series of conditional shuttle vectors for targeted genomic integration in budding yeast.
Article in FEMS yeast research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Minimal shuttle vectors forSynthetic biology (Oxford, England) · 2025Article
- Selective retention of dysfunctional mitochondria during asymmetric cell division in yeast.PLoS biology · 2023Article
- Transcription-Driven Translocation of Cohesive and Non-Cohesive Cohesin In Vivo.Molecular and cellular biology · 2023Article
- Haploinsufficiency of the sex-determining genes atiScience · 2022Article
- Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase.G3 (Bethesda, Md.) · 2021Article
- Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.PLoS genetics · 2021Article
- Genomic integration of unclonable gene expression cassettes in Saccharomyces cerevisiae using rapid cloning-free workflows.MicrobiologyOpen · 2020Article
- Yeast Expression Systems: Overview and Recent Advances.Molecular biotechnology · 2019Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The capacity of Saccharomyces cerevisiae to repair exposed DNA ends by homologous recombination has long been used by experimentalists to assemble plasmids from DNA fragments in vivo. While this approach works well for engineering extrachromosomal vectors, it is not well suited to the generation, recovery and reuse of integrative vectors. Here, we describe the creation of a series of conditional centromeric shuttle vectors, termed pXR vectors, that can be used for both plasmid assembly in vivo and targeted genomic integration. The defining feature of pXR vectors is that the DNA segment bearing the centromere and origin of replication, termed CEN/ARS, is flanked by a pair of loxP sites. Passaging the vectors through bacteria that express Cre recombinase reduces the loxP-CEN/ARS-loxP module to a single loxP site, thereby eliminating the ability to replicate autonomously in yeast. Each vector also contains a selectable marker gene, as well as a fragment of the HO locus, which permits targeted integration at a neutral genomic site. The pXR vectors provide a convenient and robust method to assemble DNAs for targeted genomic modifications.
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