ArticleJournal of the American Chemical Society2015
Conditional control of alternative splicing through light-triggered splice-switching oligonucleotides.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Optogenetic Tools for Regulating RNA Metabolism and Functions.Chembiochem : a European journal of chemical biology · 2024Review
- Direct Activation of Nucleobases with Small Molecules for the Conditional Control of Antisense Function.Angewandte Chemie (International ed. in English) · 2024Article
- Inhibition of angiogenesis and regenerative lung growth inFrontiers in cardiovascular medicine · 2024Article
- Photochemical modifications for DNA/RNA oligonucleotides.RSC advances · 2022Review
- In Situ Imaging of mRNA Splicing Variants by SpliceRCA.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- Chemical synthesis of stimuli-responsive guide RNA for conditional control of CRISPR-Cas9 gene editing.Chemical science · 2021Article
- Light-induced modulation of DNA recognition by the Rad4/XPC damage sensor protein.RSC chemical biology · 2021Article
- Light-control of cap methylation and mRNA translationChemical science · 2021Article
- Conditional Deoxyribozyme-Nanoparticle Conjugates for miRNA-Triggered Gene Regulation.ACS applied materials & interfaces · 2020Article
- SpliceRCA:ACS central science · 2018Article
- Optochemical Control of Biological Processes in Cells and Animals.Angewandte Chemie (International ed. in English) · 2018Review
- Hydrogen peroxide-triggered gene silencing in mammalian cells through boronated antisense oligonucleotides.Chemical science · 2018Article
- Stimuli-responsive oligonucleotides in prodrug-based approaches for gene silencing.Beilstein journal of organic chemistry · 2018Review
- Mammalian synthetic biology in the age of genome editing and personalized medicine.Current opinion in chemical biology · 2017Review
- RNA splicing process analysis for identifying antisense oligonucleotide inhibitors with padlock probe-based isothermal amplification.Chemical science · 2017Article
- Genetic Code Expansion in Zebrafish Embryos and Its Application to Optical Control of Cell Signaling.Journal of the American Chemical Society · 2017Article
- Sequential Uncaging with Green Light can be Achieved by Fine-Tuning the Structure of a Dicyanocoumarin Chromophore.ChemistryOpen · 2017Article
- Mammalian synthetic biology for studying the cell.The Journal of cell biology · 2017Review
- Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.Angewandte Chemie (International ed. in English) · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The spliceosome machinery is composed of several proteins and multiple small RNA molecules that are involved in gene regulation through the removal of introns from pre-mRNAs in order to assemble exon-based mRNA containing protein-coding sequences. Splice-switching oligonucleotides (SSOs) are genetic control elements that can be used to specifically control the expression of genes through correction of aberrant splicing pathways. A current limitation with SSO methodologies is the inability to achieve conditional control of their function paired with high spatial and temporal resolution. We addressed this limitation through site-specific installation of light-removable nucleobase-caging groups as well as photocleavable backbone linkers into synthetic SSOs. This enables optochemical OFF → ON and ON → OFF switching of their activity and thus precise control of alternative splicing. The use of light as a regulatory element allows for tight spatial and temporal control of splice switching in mammalian cells and animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.