Evidence map›Paper›PMID 25734547›Full record

ArticleCellular immunology2015

Monocyte-derived dendritic cells from cirrhotic patients retain similar capacity for maturation/activation and antigen presentation as those from healthy subjects.

Shiroh Tanoue, Li-Yuan Chang, Yonghai Li, David E Kaplan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Cirrhosis-associated immune dysfunction.Nature reviews. Gastroenterology & hepatology · 2022
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shiroh TanoueMedicine and Research Services, Philadelphia VA Medical Center, 3900 Woodland Avenue, Philadelphia, PA 19104, USA; Division of Gastroenterology, Department of Medicine, University of Pennsylvania, 914 BRB, 421 Curie Blvd., Philadelphia, PA 19104, USA.
Li-Yuan ChangMedicine and Research Services, Philadelphia VA Medical Center, 3900 Woodland Avenue, Philadelphia, PA 19104, USA; Division of Gastroenterology, Department of Medicine, University of Pennsylvania, 914 BRB, 421 Curie Blvd., Philadelphia, PA 19104, USA.
Yonghai LiMedicine and Research Services, Philadelphia VA Medical Center, 3900 Woodland Avenue, Philadelphia, PA 19104, USA; Division of Gastroenterology, Department of Medicine, University of Pennsylvania, 914 BRB, 421 Curie Blvd., Philadelphia, PA 19104, USA.
David E KaplanMedicine and Research Services, Philadelphia VA Medical Center, 3900 Woodland Avenue, Philadelphia, PA 19104, USA; Division of Gastroenterology, Department of Medicine, University of Pennsylvania, 914 BRB, 421 Curie Blvd., Philadelphia, PA 19104, USA. Electronic address: dakaplan@mail.med.upenn.edu.

Funding

TRANSGENIC AND CHIMERIC MOUSE COREP30DK050306 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI REBECCA G WELLS · 1997 to 2026
$32.5M
Tumor antigen-specific T-cells and hepatocellular carcinomaR01CA166111 · NCI · UNIVERSITY OF PENNSYLVANIA · PI KAPLAN, DAVID E · 2013 to 2016
$1.3M
B-Cell Dysregulation in Cirrhosis due to Chronic Hepatitis C InfectionI01CX000981 · VA · PHILADELPHIA VA MEDICAL CENTER · PI KAPLAN, DAVID E · 2014 to 2019
–
CSRD VA I01 CX000981NCI NIH HHS R01 CA166111NIDDK NIH HHS P30 DK050306
6 · The paper itself

Abstract

unlabelledFew studies have investigated the impact of liver cirrhosis on dendritic cell function. The purpose of this study was to compare the activation and antigen-presentation capacity of monocyte-derived dendritic cells (MoDC) from cirrhotic patients (CIR) relative to healthy donors (HD). MoDC from CIR and HD were matured, phenotyped, irradiated and pulsed with 15mer peptides for two hepatocellular carcinoma-related antigens, alphafetoprotein and glypican-3, then co-cultured with autologous T-cells. Expanded T-cells were evaluated by interferon-gamma ELISPOT and intracellular staining. 15 CIR and 7 HD were studied. While CD14+ monocytes from CIR displayed enhanced M2 polarization, under MoDC-polarizing conditions, we identified no significant difference between HD and CIR in maturation-induced upregulation of co-stimulation markers. Furthermore, no significant differences were observed between CIR and HD in subsequent expansion of tumor antigen-specific IFNγ+ T-cells.

conclusionMoDCs isolated from cirrhotic individuals retain similar capacity for in vitro activation, maturation and antigen-presentation as those from healthy donors.

Indexed as

AdultAgedAntigen PresentationCell DifferentiationCell ProliferationCells, CulturedCoculture TechniquesDendritic CellsEnzyme-Linked Immunospot AssayFlow CytometryHealthy VolunteersHumansImmunophenotypingInterferon-gammaLipopolysaccharide ReceptorsLiver CirrhosisInterferon-gammaLipopolysaccharide ReceptorsCirrhosisDendritic cellGlypican-3Hepatitis CHumanM1 macrophageM2 macrophageMonocyte

Identifiers

PMID25734547
PMCPMC4405471

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.