ArticleBiochemistry insights2015
Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System.
Article in Biochemistry insights, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Production and statistical optimization of cholesterol-oxidase generated by Streptomyces sp. AN strain.Journal, genetic engineering & biotechnology · 2022Article
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- Whole genome sequencing analysis of a dexamethasone-degrading Burkholderia strain CQ001.Medicine · 2019Article
- Bioprospecting of whole-cell biocatalysts for cholesterol biotransformation.World journal of microbiology & biotechnology · 2019Article
- Cholesterol Degradation and Production of Extracellular Cholesterol Oxidase fromBioMed research international · 2019Article
- Anti-BACE1 and Antimicrobial Activities of Steroidal Compounds Isolated from Marine Urechis unicinctus.Marine drugs · 2018Article
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- Simultaneous Determination of Six Active Components inJournal of analytical methods in chemistry · 2017Article
- Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system.Journal of Zhejiang University. Science. BArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD) using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus) instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product.
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Registered trials
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