ArticleProceedings of the National Academy of Sciences of the United States of America2015
Improving recombinant Rubisco biogenesis, plant photosynthesis and growth by coexpressing its ancillary RAF1 chaperone.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 132 citations in OpenAlex.
- Machine learning-assisted directed evolution of plant Rubisco.Science advances · 2026Article
- Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression.Nature biotechnology · 2026Article
- Very high CONAR genomics and bioinformatics · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Directed evolution of a plant Rubisco chaperone with altered client recognition.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Review
- Article
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- Rubisco is evolving for improved catalytic efficiency and COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth.Nature plants · 2023Article
- Towards engineering a hybrid carboxysome.Photosynthesis research · 2023Article
- Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways.Journal of advanced research · 2023Review
- Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress.Applied biochemistry and biotechnology · 2023Review
- Producing fast and active Rubisco in tobacco to enhance photosynthesis.The Plant cell · 2023Article
- Red Rubiscos and opportunities for engineering green plants.Journal of experimental botany · 2023Review
- Predicting plant Rubisco kinetics from RbcL sequence data using machine learning.Journal of experimental botany · 2023Article
- Overexpression ofGenes · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enabling improvements to crop yield and resource use by enhancing the catalysis of the photosynthetic CO2-fixing enzyme Rubisco has been a longstanding challenge. Efforts toward realization of this goal have been greatly assisted by advances in understanding the complexities of Rubisco's biogenesis in plastids and the development of tailored chloroplast transformation tools. Here we generate transplastomic tobacco genotypes expressing Arabidopsis Rubisco large subunits (AtL), both on their own (producing tob(AtL) plants) and with a cognate Rubisco accumulation factor 1 (AtRAF1) chaperone (producing tob(AtL-R1) plants) that has undergone parallel functional coevolution with AtL. We show AtRAF1 assembles as a dimer and is produced in tob(AtL-R1) and Arabidopsis leaves at 10-15 nmol AtRAF1 monomers per square meter. Consistent with a postchaperonin large (L)-subunit assembly role, the AtRAF1 facilitated two to threefold improvements in the amount and biogenesis rate of hybrid L8(A)S8(t) Rubisco [comprising AtL and tobacco small (S) subunits] in tob(AtL-R1) leaves compared with tob(AtL), despite >threefold lower steady-state Rubisco mRNA levels in tob(AtL-R1). Accompanying twofold increases in photosynthetic CO2-assimilation rate and plant growth were measured for tob(AtL-R1) lines. These findings highlight the importance of ancillary protein complementarity during Rubisco biogenesis in plastids, the possible constraints this has imposed on Rubisco adaptive evolution, and the likely need for such interaction specificity to be considered when optimizing recombinant Rubisco bioengineering in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.