ArticleProceedings of the National Academy of Sciences of the United States of America2015
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 801 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
801 citing papers in PubMed, 1 synthesis or guideline pooled it, 1,571 citations in OpenAlex.
- Pooled it
- CRISPR/Cas-based genome editing for enhancing grain quality traits in rice (Plant signaling & behavior · 2026Review
- Metabolic reprogramming in plant defense: linking signaling networks to metabolomics-driven insights.Plant signaling & behavior · 2026Review
- The OsSPL-SNB complex orchestrates the reproductive transition through tillering suppression in rice.aBIOTECH · 2026Article
- CRISPR editing of TERMINAL FLOWER 1 recapitulates perpetual flowering in strawberry, mimicking repression by the PERPETUAL FLOWERING locus.Plant physiology · 2026Article
- ZEP1 orchestrates template choice and crossover pathways to ensure meiotic genome integrity in rice.The EMBO journal · 2026Article
- Gb3 encodes a unique kinase fusion protein conferring greenbug resistance in wheat.Nature genetics · 2026Article
- Circadian- and light-regulated oscillatory expression of CSA in rice leaves is required for pollen fertility.Plant cell reports · 2026Article
- The dehydrin protein COR15 enhances antiviral RNA silencing by preventing viral protein-mediated SGS3 degradation and promoting SGS3 condensate formation.Plant communications · 2026Article
- A RUBY-Based Visual Hairy Root Transformation and CRISPR-Plants (Basel, Switzerland) · 2026Article
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing.Plant direct · 2026Article
- Technologies for engineering repetitive DNA.Quantitative biology (Beijing, China) · 2026Review
- AI-designed OpenCRISPR-1 performs robust knockout, base editing, and prime editing in rice.The New phytologist · 2026Article
- An LRR-truncated NLR natural variant confers dual resistance to drought and blast in rice.Nature communications · 2026Article
- Dpb11 facilitates the colocalization of Mec1-Ddc2 with its activators on gapped DNA.Cell reports · 2026Article
- Rice PROTEIN L-ISOASPARTYL METHYLTRANSFERASES provides tolerance against sheath blight disease and repairs ALDH and PBZ1.Nature communications · 2026Article
- CRISPR-Cas systems for enhancing chilling tolerance in rice: recent advances and future prospects.Biologia futura · 2026Review
- A two-step auxin-GA cross-talk regulates organ formation in tomato.Development (Cambridge, England) · 2026Article
- Turnip crinkle virus coat protein subverts DRB7-mediated compensatory RNAi through ubiquitin-proteasome-dependent degradation.Plant communications · 2026Article
741 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system is being harnessed as a powerful tool for genome engineering in basic research, molecular therapy, and crop improvement. This system uses a small guide RNA (gRNA) to direct Cas9 endonuclease to a specific DNA site; thus, its targeting capability is largely constrained by the gRNA-expressing device. In this study, we developed a general strategy to produce numerous gRNAs from a single polycistronic gene. The endogenous tRNA-processing system, which precisely cleaves both ends of the tRNA precursor, was engineered as a simple and robust platform to boost the targeting and multiplex editing capability of the CRISPR/Cas9 system. We demonstrated that synthetic genes with tandemly arrayed tRNA-gRNA architecture were efficiently and precisely processed into gRNAs with desired 5' targeting sequences in vivo, which directed Cas9 to edit multiple chromosomal targets. Using this strategy, multiplex genome editing and chromosomal-fragment deletion were readily achieved in stable transgenic rice plants with a high efficiency (up to 100%). Because tRNA and its processing system are virtually conserved in all living organisms, this method could be broadly used to boost the targeting capability and editing efficiency of CRISPR/Cas9 toolkits.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.