Evidence map›Paper›PMID 25733783›Full record

ArticleEthiopian journal of health sciences2015

Human rotavirus group a serotypes causing gastroenteritis in children less than 5 years and HIV-infected adults in Viwandani slum, Nairobi.

S K Raini, J Nyangao, J Kombich, C Sang, J Gikonyo, J R Ongus, E O Odari

Open access · diamondAbstract read
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Article in Ethiopian journal of health sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 3 pooled it
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 3 syntheses or guidelines pooled it, 17 citations in OpenAlex.

  1. Pooled it
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  7. Cynomolgus Monkeys (Viruses · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

S K RainiCollege of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
J NyangaoCentre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
J KombichCentre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
C SangCollege of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
J GikonyoCentre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
J R OngusCollege of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
E O OdariCollege of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya ; Max von Pettenkofer-Institute, Munich, Germany.
Jomo Kenyatta University of Agriculture and Technology · KEKenya Medical Research Institute · KE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRotavirus remains a leading cause of acute gastroenteritis in children worldwide with an estimated 2000 deaths each day in developing countries. Due to HIV/AIDS scourge in Kenya, it is possible that rotavirus-related gastroenteritis has been aggravated in adults. The Global Alliance for Immunizations has ranked rotavirus infection a priority for vaccine, and, to ensure its success, there is a need to document the local strain(s) circulating in different regions.

methodsA cross-sectional study was conducted to document human rotavirus group A serotypes in children below 5 years and HIV-infected adults in Viwandani slum in Nairobi, Kenya. A total of 260 (128 from children and 132 from HIV infected adults) fecal specimen samples were analyzed from August 2012 to July 2013. Screening for rotavirus was done by antigen based enzyme immune-sorbent assay (ELISA), Polyacrylamide gel electrophoresis (PAGE) was used to detect rotavirus electropherotypes and finally genotyping was done by RT-PCR using genotype-specific primer sets targeting VP4 and VP7 genes.

resultsRotavirus was detected in 23% and 8% of children and adult, respectively. Prevalence was high in children of < 2 years and adults of > 48 years. Long electropherotypes accounted for 80% and 60% while short electropherotypes accounted for 20% and 40% in children and adult, respectively. The common globally distributed strains, G1 and G3, accounted for 60% detections while the unusual G9 strain accounted for 80% infection in adults. G1P[8] was the common genotypic combination in children, accounting for 40% infection, whereas G9 [P8] accounted for 60% of the infections in adults.

conclusionThis study shows the existence of strain diversity between rotavirus circulating in children and adults within this study group. It further shows that as currently constituted, the 2 vaccines recommended for rotavirus would cover the circulating strain in Viwandani slum. Finally, there is a need for continuous rotavirus strain surveillance in children and a further focus on HIV infected adults.

Indexed as

GenotypePoverty AreasSerogroupAdultChild, PreschoolCross-Sectional StudiesFemaleGastroenteritisHIV InfectionsHumansInfantKenyaMalePrevalenceRotavirusRotavirus InfectionsDiarrhoeaGastroenteritisKenyaRotavirusViwandani slum

Identifiers

PMID25733783
PMCPMC4337073
OpenAlexW1986021040

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.